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UBE2H Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515398

Recombinant full length human UBE2H was expressed in E. coli cells using an N-terminal His tag.Scientific Background: UBE2H or ubiquitin-conjugating enzyme E2H is a member of the E2 ubiquitin-conjugating enzyme family which is located on chromosome 7 (1).… More details

$255 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
UBE2H, Recombinant Protein
Full Product Name
UBE2H recombinant protein
Product Gene Name
Product Synonym Gene Name
E2-20K; UBC8; UBCH; UBCH2
Product Synonym Names
E2-20K; UBC8; UBCH; UBCH2; UBE2H protein
Host
E Coli
Form/Format
50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
~21 kDa
Chromosome Location
Chromosome: 7; NC_000007.13 (129470572..129592800, complement). Location: 7q32
OMIM
601082
3D Structure
ModBase 3D Structure for P62256
Type
Recombinant Fusion Protein
Species
Human
Tag Information
His tag
Expression System
E.coli
Source Note
Recombinant full length human UBE2H was expressed in E. coli cells
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Applications Tested/Suitable for UBE2H recombinant protein
Western Blot (WB)

Product References and Citations for UBE2H recombinant protein

1. Kaiser, P. et.al: Human ubiquitin-conjugating enzyme homologous to yeast UBC8. J. Biol. Chem. 269: 8797-8802, 1994.
2. Lausen J,Targets of the Tal1 transcription factor in erythrocytes: E2 ubiquitin conjugase regulation by Tal1. J Biol Chem. 2010 Feb 19;285(8):5338-46.

Other Notes

Small volumes of UBE2H recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UBE2H purchase

MBS515398 is a recombinant protein targeting UBE2H. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for UBE2H. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens ubiquitin-conjugating enzyme E2H (UBE2H), transcript variant 1, mRNA
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2H
NCBI Official Symbol
UBE2H
NCBI Official Synonym Symbols
GID3; UBC8; UBCH; UBCH2; E2-20K
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 H
UniProt Synonym Protein Names
UbcH2; Ubiquitin carrier protein H; Ubiquitin-conjugating enzyme E2-20K; Ubiquitin-protein ligase H
UniProt Gene Name
UBE2H
UniProt Entry Name
UBE2H_HUMAN
NCBI Protein Information
ubiquitin-conjugating enzyme E2 H; ubiquitin-protein ligase H; ubiquitin carrier protein H; GID complex subunit 3, UBC8 homolog; ubiquitin-conjugating enzyme E2-20K; ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast); ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8)

NCBI Summary for UBE2H

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq, Feb 2011]
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UniProt Comments for UBE2H

Function: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A. Ref.1 Ref.8

Catalytic activity: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway: Protein modification; protein ubiquitination.

Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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