RhoD Recombinant Protein

Name: RhoD Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS515432
Price: 300 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515432

Recombinant full length human RhoD was expressed in E. coli cells using an N-terminal His tag.Scientific Background: RhoD is a member of the Rho gene family that interacts with protein kinases and serves as a target of activated GTPase. Rho binds GTP and… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

RhoD, Recombinant Protein

Full Product Name

RhoD recombinant protein

Product Gene Name

RhoD recombinant protein

Product Synonym Gene Name

Rho; RHOHP1; RHOM

Product Synonym Names

Rho; RHOHP1; RHOM

Host

E Coli

Form/Format

50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

Preparation and Storage

Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Molecular Weight

~22 kDa

Chromosome Location

Chromosome: 11; NC_000011.9 (66824289..66839488). Location: 11q14.3

OMIM

605781

3D Structure

ModBase 3D Structure for O00212

Type

Recombinant Fusion Protein

Species

Human

Tag Information

His tag

Expression System

E.coli

Source Note

Recombinant full length human RhoD was expressed in E. coli cells

Product Categories/Family for RhoD recombinant protein

G-Proteins; Signaling Proteins - G-Proteins

Applications Tested/Suitable for RhoD recombinant protein

Kinase Assay, Western Blot (WB)

Product References and Citations for RhoD recombinant protein

1. Gasman, S. et al: RhoD regulates endosome dynamics through Diaphanous-related Formin and Src tyrosine kinase. Nature Cell Biol. 5: 195-204, 2003.
2. Narumiya, S. et al: Rho signaling, ROCK and mDia1, in transformation, metastasis and invasion. Cancer Metastasis Rev. 2009 Jun;28(1-2):65-76.

Other Notes

Small volumes of RhoD recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RhoD purchase

MBS515432 is a recombinant protein targeting RhoD. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for RhoD. It may not necessarily be applicable to this product.

NCBI GI #

12654980

NCBI GeneID

29984

NCBI Accession #

BC001338

UniProt Primary Accession #

O00212

UniProt Related Accession #

O00212

NCBI Official Full Name

Homo sapiens ras homolog gene family, member D, mRNA

NCBI Official Synonym Full Names

ras homolog family member D

NCBI Official Symbol

RHOD

NCBI Official Synonym Symbols

Rho; ARHD; RHOM; RHOHP1

UniProt Protein Name

Rho-related GTP-binding protein RhoD

UniProt Synonym Protein Names

Rho-related protein HP1

Protein Family

Rho-related GTP-binding protein

UniProt Gene Name

RHOD

UniProt Synonym Gene Names

ARHD; RhoHP1

UniProt Entry Name

RHOD_HUMAN

NCBI Protein Information

rho-related GTP-binding protein RhoD; ras homolog D; Rho-related protein HP1; ras homolog gene family, member A; ras homolog gene family, member D

NCBI Summary for RhoD

Ras homolog, or Rho, proteins interact with protein kinases and may serve as targets for activated GTPase. They play a critical role in muscle differentiation. The protein encoded by this gene binds GTP and is a member of the small GTPase superfamily. It is involved in endosome dynamics and reorganization of the actin cytoskeleton, and it may coordinate membrane transport with the function of the cytoskeleton. [provided by RefSeq, Jul 2008]

UniProt Comments for RhoD

Function: Involved in endosome dynamics. May coordinate membrane transport with the function of the cytoskeleton. Participates in reorganization of actin cytoskeleton

By similarity.

Subunit structure: In the GTP-bound form, interacts with DIAPH2 isoform 3. Interacts with PAK7/PAK5. Ref.5 Ref.6

Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic side

Potential.

Tissue specificity: Heart, placenta, liver, skeletal muscle, and pancreas and, with weaker intensity, in several other tissues.

Sequence similarities: Belongs to the small GTPase superfamily. Rho family.

Research Articles on RhoD

1. Data from differentiating cultured erythroid precursor cells suggest that RhAG (Rh-associated glycoprotein) knockdown abolishes Rh blood group expression (RhoD; ICAM4 [intercellular adhesion molecule 4]; CD47 Rh-related antigen) in erythroid cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with RhoD recombinant protein

Reference Product	PubMed Publications
PLXNA1 recombinant protein	>1 publications with RhoD and PLXNA1

Pathways associated with RhoD recombinant protein

Products by Pathway	Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Rho GTPase Cycle Pathway antibodies	Rho GTPase Cycle Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By Rho GTPases Pathway antibodies	Signaling By Rho GTPases Pathway Diagram

Diseases associated with RhoD recombinant protein

Disease Name	Pubmed Publications
Necrosis Antibodies	>11 publications with RhoD and Necrosis
Inflammation Antibodies	>7 publications with RhoD and Inflammation
Carcinoma Antibodies	>5 publications with RhoD and Carcinoma
Blood Coagulation Disorders Antibodies	>3 publications with RhoD and Blood Coagulation Disorders
Liver Diseases Antibodies	>3 publications with RhoD and Liver Diseases
Neoplasm Metastasis Antibodies	>3 publications with RhoD and Neoplasm Metastasis
Adenocarcinoma Antibodies	>3 publications with RhoD and Adenocarcinoma
Hemorrhage Antibodies	>3 publications with RhoD and Hemorrhage
Kidney Neoplasms Antibodies	>2 publications with RhoD and Kidney Neoplasms
Fatty Liver Antibodies	>2 publications with RhoD and Fatty Liver

Organs/Tissues associated with RhoD recombinant protein

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>122 publications with RhoD and Muscle
Heart Antibodies	>105 publications with RhoD and Heart
Eye Antibodies	>22 publications with RhoD and Eye
Kidney Antibodies	>13 publications with RhoD and Kidney
Liver Antibodies	>9 publications with RhoD and Liver
Pancreas Antibodies	>8 publications with RhoD and Pancreas
Lung Antibodies	>7 publications with RhoD and Lung
Embryonic Tissue Antibodies	>6 publications with RhoD and Embryonic Tissue
Bone Antibodies	>6 publications with RhoD and Bone
Intestine Antibodies	>6 publications with RhoD and Intestine