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KIAA1549-BRAF Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515457

Description: Recombinant human fusion KIAA1549 (1320-1749 exon16) -BRAF (381-end or exon9-18) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: KIAA1549-BRAF is a gene fusion resulting from a tandem… More details

$480 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
KIAA1549-BRAF, Recombinant Protein
Full Product Name
KIAA1549-BRAF Mutant (Kex16Bex9), Active
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
~125 kDa
Sequence Positions
1320-1749 exon16 (KIAA1549) - 381-end or exon9-18 (BRAF)
OMIM
AM989467 Genomic DNA
3D Structure
ModBase 3D Structure for Q9HCM3
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Applications Tested/Suitable for KIAA1549-BRAF recombinant protein
Kinase Assay

Product References and Citations for KIAA1549-BRAF recombinant protein

1. Dougherty, M J. et al: Activating mutations in BRAF characterize a spectrum of pediatric low-grade gliomas. Neuro Oncol. 2010 Jul;12 (7):621-30. 2. Badiali, M. et al: KIAA1549-BRAF fusions and IDH mutations can coexist in diffuse gliomas of adults. Brain Pathol. 2012 Nov;22 (6):841-7.

Other Notes

Small volumes of KIAA1549-BRAF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KIAA1549-BRAF purchase

MBS515457 is a recombinant protein targeting KIAA1549-BRAF. Also known as KIAA1549-BRAF Mutant (Kex16Bex9), Active. Host: Sf9 insect cells using baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for KIAA1549-BRAF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens KIAA1549 (KIAA1549), transcript variant 2, mRNA
NCBI Official Synonym Full Names
KIAA1549
NCBI Official Symbol
KIAA1549
UniProt Protein Name
UPF0606 protein KIAA1549
UniProt Gene Name
KIAA1549
NCBI Protein Information
UPF0606 protein KIAA1549

NCBI Summary for KIAA1549-BRAF

The protein encoded by this gene belongs to the UPF0606 family. This gene has been found to be fused to the BRAF oncogene in many cases of pilocytic astrocytoma. The fusion results from 2Mb tandem duplications at 7q34. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]
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UniProt Comments for KIAA1549-BRAF

KIAA1549: A chromosomal aberration involving KIAA1549 is found in pilocytic astrocytoma. A tandem duplication of 2 Mb at 7q34 leads to the expression of a KIAA1549-BRAF fusion protein with a constitutive kinase activity and inducing cell transformation. Belongs to the UPF0606 family. 4 isoforms of the human protein are produced by alternative promoter.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: plasma membrane
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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