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RNF130-BRAF Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515460

Description: Recombinant human fusion RNF130 (1-231 exon3)-BRAF (381-end or exon9-18) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: KIAA1549-BRAF is a gene fusion resulting from a tandem… More details

$480 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RNF130-BRAF, Recombinant Protein
Full Product Name
RNF130-BRAF Mutant (Rex3Bex9), Active
Product Synonym Gene Name
G1RZFP; GOLIATH; GP
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
~98 kDa
Sequence Positions
1-231 exon3 (RNF130) - 381-end or exon 9-18 (BRAF)
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Applications Tested/Suitable for RNF130-BRAF recombinant protein
Kinase Assay

Product References and Citations for RNF130-BRAF recombinant protein

1. Dougherty, M J. et al: Activating mutations in BRAF characterize a spectrum of pediatric low-grade gliomas. Neuro Oncol. 2010 Jul;12 (7):621-30. 2. Badiali, M. et al: KIAA1549-BRAF fusions and IDH mutations can coexist in diffuse gliomas of adults. Brain Pathol. 2012 Nov;22 (6):841-7.

Other Notes

Small volumes of RNF130-BRAF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RNF130-BRAF purchase

MBS515460 is a recombinant protein targeting RNF130-BRAF. Also known as RNF130-BRAF Mutant (Rex3Bex9), Active. Host: Sf9 insect cells using baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for RNF130-BRAF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Homo sapiens ring finger protein 130, mRNA
NCBI Official Synonym Full Names
ring finger protein 130
NCBI Official Symbol
RNF130
NCBI Official Synonym Symbols
GP; G1RZFP; GOLIATH
UniProt Protein Name
E3 ubiquitin-protein ligase RNF130
UniProt Synonym Protein Names
Goliath homolog; H-Goliath; RING finger protein 130; RING-type E3 ubiquitin transferase RNF130Curated
UniProt Gene Name
RNF130
UniProt Synonym Gene Names
H-Goliath
NCBI Protein Information
E3 ubiquitin-protein ligase RNF130

NCBI Summary for RNF130-BRAF

The protein encoded by this gene contains a RING finger motif and is similar to g1, a Drosophila zinc-finger protein that is expressed in mesoderm and involved in embryonic development. The expression of the mouse counterpart was found to be upregulated in myeloblastic cells following IL3 deprivation, suggesting that this gene may regulate growth factor withdrawal-induced apoptosis of myeloid precursor cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
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UniProt Comments for RNF130-BRAF

RNF130: May have a role during the programmed cell death of hematopoietic cells. Acts as an E3 ubiquitin- protein ligase.

Protein type: EC 6.3.2.-; EC 6.3.2.19; Ligase; Membrane protein, integral; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: cytoplasm

Molecular Function: ubiquitin-protein ligase activity

Biological Process: programmed cell death
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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