CDK1/Cyclina1 (CDK1) Recombinant Protein

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

CDK1: a protein kinase of the CDK family. Catalytic subunit of the conserved protein complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions. Mitotic cyclins stably associate with this protein and function as regulatory subunits. Its activity is controlled by cyclin availability and phosphorylation through the cell cycle. Activated in many cancers including colon, liver and breast. The T isoform, which lacks a regulatory region, is expressed in breast cancer. Inhibition in cancer cells may drive cells into apoptosis. May also drive cell migration. Inhibitors: BMS-265246, BMS-265246-01. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: CDK family; CDK/CDK1 subfamily; CDK1 subfamily; CMGC group; Cell cycle regulation; EC 2.7.11.22; EC 2.7.11.23; Kinase, protein; Motility/polarity/chemotaxis; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 10q21.2

Cellular Component: centrosome; cyclin-dependent protein kinase holoenzyme complex; cytosol; endoplasmic reticulum membrane; membrane; midbody; mitochondrial matrix; mitochondrion; nuclear chromosome, telomeric region; nucleoplasm; nucleus; spindle microtubule

Molecular Function: cyclin binding; cyclin-dependent protein kinase activity; histone kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity; RNA polymerase subunit kinase activity

Biological Process: activation of MAPK activity; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell migration; centrosome cycle; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA repair; DNA replication; epithelial cell differentiation; G2/M transition of mitotic cell cycle; microtubule cytoskeleton organization and biogenesis; mitotic nuclear envelope disassembly; negative regulation of apoptosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; pronuclear fusion; proteasomal ubiquitin-dependent protein catabolic process; protein amino acid phosphorylation; protein deubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of embryonic development; regulation of gene expression; regulation of meiotic cell cycle; regulation of Schwann cell differentiation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle