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LCMT1 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515535

Description: Recombinant full-length human LCMT1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: Leucine carboxyl methyltransferase 1 (LCMT1) exclusively methylates the carboxyl group of leucine-309 in the… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LCMT1, Recombinant Protein
Full Product Name
LCMT1, Active
Product Gene Name
LCMT1 recombinant protein
Product Synonym Gene Name
CGI-68; LCMT; PPMT1
Host
Sf9 insect cells using baculovirus
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
63 kDa
Sequence Positions
Full length
OMIM
AF037601 mRNA
3D Structure
ModBase 3D Structure for Q9UIC8
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for LCMT1 recombinant protein

Enzymes; Active Methyltransferases
Applications Tested/Suitable for LCMT1 recombinant protein
Methyltransferase Assay

Testing Data of LCMT1 recombinant protein

LCMT1 Recombinant Protein – Cat# MBS515535

Testing Data #2 of LCMT1 recombinant protein

LCMT1 Recombinant Protein, Testing Data #2 – Cat# MBS515535

Product References and Citations for LCMT1 recombinant protein

1. De Baere, I., et al. Purification of porcine brain protein phosphatase 2A leucine carboxyl methyltransferase and cloning of the human homologue. Biochemistry. 38 (50):16539-47, 1999. 2. Lee, J.A., et al. Leucine carboxyl methyltransferase-1 is necessary for normal progression through mitosis in mammalian cells. J Biol Chem. 282 (42):30974-84, 2007. 3. Sontag, J.M., et al. Leucine carboxyl methyltransferase 1 (LCMT1)-dependent methylation regulates the association of protein phosphatase 2A and Tau protein with plasma membrane microdomains in neuroblastoma cells. J Biol Chem. 288 (38):27396-405, 2013.

Other Notes

Small volumes of LCMT1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LCMT1 purchase

MBS515535 is a recombinant protein targeting LCMT1. Also known as LCMT1, Active. Host: Sf9 insect cells using baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for LCMT1. It may not necessarily be applicable to this product.

NCBI GI #
74048433
NCBI GeneID
51451
NCBI Accession #
NM_016309.2
UniProt Primary Accession #
Q9UIC8
UniProt Secondary Accession #
Q53FC5; Q96CI5; Q9H6I9; Q9NTG4; Q9Y378; A6NL89; A8K770
UniProt Related Accession #
Q9UIC8
GenBank Nucleotide #
NM_016309
NCBI Official Full Name
Homo sapiens leucine carboxyl methyltransferase 1 (LCMT1), transcript variant 1, mRNA
NCBI Official Synonym Full Names
leucine carboxyl methyltransferase 1
NCBI Official Symbol
LCMT1
NCBI Official Synonym Symbols
LCMT; PPMT1; CGI-68
UniProt Protein Name
Leucine carboxyl methyltransferase 1
UniProt Synonym Protein Names
Protein-leucine O-methyltransferase; [Phosphatase 2A protein]-leucine-carboxy methyltransferase 1
Protein Family
Leucine carboxyl methyltransferase
UniProt Gene Name
LCMT1
UniProt Synonym Gene Names
LCMT
NCBI Protein Information
leucine carboxyl methyltransferase 1

NCBI Summary for LCMT1

LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A (PPP2CA; MIM 176915) (De Baere et al., 1999 [PubMed 10600115]).[supplied by OMIM, Mar 2008]

UniProt Comments for LCMT1

LCMT1: Methylates the carboxyl group of the C-terminal leucine residue of protein phosphatase 2A catalytic subunits to form alpha-leucine ester residues. Belongs to the methyltransferase superfamily. LCMT family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - histidine; Amino Acid Metabolism - tyrosine; EC 2.1.1.233; Lipid Metabolism - androgen and estrogen; Methyltransferase; Other Amino Acids Metabolism - selenoamino acid

Chromosomal Location of Human Ortholog: 16p12.1

Cellular Component: cytosol; nucleoplasm

Molecular Function: C-terminal protein carboxyl methyltransferase activity; protein binding; protein-leucine O-methyltransferase activity; S-adenosylmethionine-dependent methyltransferase activity

Biological Process: C-terminal protein amino acid methylation; G2/M transition of mitotic cell cycle; negative regulation of protein complex assembly; protein amino acid methylation; protein modification process; regulation of apoptosis
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Research Articles on LCMT1

1. LCMT1-PME-1 methylation equilibrium is critical for regulating mitotic spindle size and thereby proper cell division

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with LCMT1 recombinant protein

 Reference Product  PubMed Publications
 CASP3 recombinant protein  >1 publications with LCMT1 and CASP3
 PPP2R1B recombinant protein  >1 publications with LCMT1 and PPP2R1B
 PPP2R4 recombinant protein  >1 publications with LCMT1 and PPP2R4
 PPME1 recombinant protein  >1 publications with LCMT1 and PPME1
 PPP2CA recombinant protein  >1 publications with LCMT1 and PPP2CA
 PPP2R2A recombinant protein  >1 publications with LCMT1 and PPP2R2A

Diseases associated with LCMT1 recombinant protein

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >1 publications with LCMT1 and Disease Models, Animal
 Embryo Loss Antibodies  >1 publications with LCMT1 and Embryo Loss

Organs/Tissues associated with LCMT1 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >5 publications with LCMT1 and Brain
 Vascular Antibodies  >1 publications with LCMT1 and Vascular
 Embryonic Tissue Antibodies  >1 publications with LCMT1 and Embryonic Tissue
 Pancreas Antibodies  >1 publications with LCMT1 and Pancreas
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