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NNMT Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515561

Description: Recombinant full-length human NNMT was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: Nicotinamide N-methyltransferase (NNMT) is expressed predominantly in the liver, and mediates the N-methylation… More details

$505 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NNMT, Recombinant Protein
Full Product Name
NNMT, Active
Product Gene Name
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
54 kDa
Sequence Positions
Full length
OMIM
U08021 mRNA
3D Structure
ModBase 3D Structure for P40261
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Applications Tested/Suitable for NNMT recombinant protein
Methyltransferase Assay

Testing Data of NNMT recombinant protein

NNMT Recombinant Protein – Cat# MBS515561

Testing Data #2 of NNMT recombinant protein

NNMT Recombinant Protein, Testing Data #2 – Cat# MBS515561

Product References and Citations for NNMT recombinant protein

1. Aksoy, S., et al. Human liver nicotinamide N-methyl-transferase. cDNA cloning, expression, and biochemical characterization. J Biol Chem. 269 (20):14835-40, 1994. 2. Roessler, M., et al. Identification of nicotinamide N-methyl-transferase as a novel serum tumor marker for colorectal cancer. Clin Cancer Res. 11 (18):6550-7, 2005. 3. Sartini, D., et al. Upregulation of tissue and urinary nicotinamide N-methyltransferase in bladder cancer: potential for the development of a urine-based diagnostic test. Cell Biochem Biophys. 65 (3):473-83, 2013.

Other Notes

Small volumes of NNMT recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NNMT purchase

MBS515561 is a recombinant protein targeting NNMT. Also known as NNMT, Active. Host: Sf9 insect cells using baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for NNMT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens nicotinamide N-methyltransferase (NNMT), mRNA
NCBI Official Synonym Full Names
nicotinamide N-methyltransferase
NCBI Official Symbol
NNMT
UniProt Protein Name
Nicotinamide N-methyltransferase
UniProt Gene Name
NNMT
NCBI Protein Information
nicotinamide N-methyltransferase

NCBI Summary for NNMT

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq, Jul 2008]

UniProt Comments for NNMT

NNMT: Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. Belongs to the NNMT/PNMT/TEMT family.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 2.1.1.1; Methyltransferase

Chromosomal Location of Human Ortholog: 11q23.2

Cellular Component: cytosol

Molecular Function: nicotinamide N-methyltransferase activity; pyridine N-methyltransferase activity

Biological Process: methylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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