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PI4K2A Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515576

Description: Recombinant full-length human PI4K2A was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: PI4K2A or phosphatidylinositol 4-kinase type 2 alpha is involved in the formation of… More details

$505 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PI4K2A, Recombinant Protein
Full Product Name
PI4K2A, Active
Product Gene Name
PI4K2A recombinant protein
Product Synonym Gene Name
DKFZp761G1923; PI4KII; PIK42A; RP11-548K23.6
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
~79 kDa
Sequence Positions
Full length
OMIM
AJ303098 mRNA
3D Structure
ModBase 3D Structure for Q9BTU6
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for PI4K2A recombinant protein

Enzymes; Active Lipid Kinases
Applications Tested/Suitable for PI4K2A recombinant protein
Kinase Assay

Testing Data of PI4K2A recombinant protein

PI4K2A Recombinant Protein – Cat# MBS515576

Testing Data #2 of PI4K2A recombinant protein

PI4K2A Recombinant Protein, Testing Data #2 – Cat# MBS515576

Product References and Citations for PI4K2A recombinant protein

1. Wang. et.al: Phosphatidylinositol 4 phosphate regulates targeting of clathrin adaptor AP-1 complexes to the Golgi. Cell 114: 299-310, 2003. 2. Pan. et.al: Wnt3a-mediated formation of phospha-tidylinositol 4,5-bisphosphate regulates LRP6 phospho-rylation. Science 321: 1350-1353, 2008.

Other Notes

Small volumes of PI4K2A recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PI4K2A purchase

MBS515576 is a recombinant protein targeting PI4K2A. Also known as PI4K2A, Active. Host: Sf9 insect cells using baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for PI4K2A. It may not necessarily be applicable to this product.

NCBI GI #
734520304
NCBI GeneID
55361
NCBI Accession #
NM_018425.3
UniProt Primary Accession #
Q9BTU6
UniProt Secondary Accession #
Q9NSG8; D3DR59
UniProt Related Accession #
Q9BTU6
GenBank Nucleotide #
NM_018425
NCBI Official Full Name
Homo sapiens phosphatidylinositol 4-kinase type 2 alpha (PI4K2A), mRNA
NCBI Official Synonym Full Names
phosphatidylinositol 4-kinase type 2 alpha
NCBI Official Symbol
PI4K2A
NCBI Official Synonym Symbols
PI4KII; PIK42A
UniProt Protein Name
Phosphatidylinositol 4-kinase type 2-alpha
UniProt Synonym Protein Names
Phosphatidylinositol 4-kinase type II-alpha
Protein Family
Phosphatidylinositol 4-kinase
UniProt Gene Name
PI4K2A
NCBI Protein Information
phosphatidylinositol 4-kinase type 2-alpha

NCBI Summary for PI4K2A

Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs (Barylko et al., 2001 [PubMed 11244087]).[supplied by OMIM, Mar 2008]
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UniProt Comments for PI4K2A

PI4K2A: Together with PI4K2B and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells. Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.

Protein type: EC 2.7.1.67; Kinase, lipid; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q24.2

Cellular Component: cell soma; cytoplasmic vesicle; cytosol; dendrite; endosome; integral to plasma membrane; intrinsic to membrane; lipid raft; lysosomal membrane; membrane; mitochondrion; neuron projection; plasma membrane; presynaptic membrane; trans-Golgi network

Molecular Function: 1-phosphatidylinositol 4-kinase activity; ATP binding; protein binding

Biological Process: endosome organization and biogenesis; Golgi organization and biogenesis; phosphatidylinositol biosynthetic process; phosphoinositide phosphorylation
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Research Articles on PI4K2A

1. PtdIns4KIIalpha regulates receptor sorting at early endosomes through a PtdIns(4)P-dependent pathway and contributes substrate for the synthesis of endosomal PtdIns(4,5)P2.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PI4K2A recombinant protein

 Reference Product  PubMed Publications
 PIP5K1C recombinant protein  >1 publications with PI4K2A and PIP5K1C

Pathways associated with PI4K2A recombinant protein

 Products by Pathway  Pathway Diagram
 3-phosphoinositide Biosynthesis Pathway antibodies  3-phosphoinositide Biosynthesis Pathway Diagram
 3-phosphoinositide Biosynthesis Pathway antibodies  3-phosphoinositide Biosynthesis Pathway Diagram
 Canonical Wnt Signaling Pathway antibodies  Canonical Wnt Signaling Pathway Diagram
 D-myo-inositol (1,4,5)-trisphosphate Biosynthesis Pathway antibodies  D-myo-inositol (1,4,5)-trisphosphate Biosynthesis Pathway Diagram
 D-myo-inositol (1,4,5)-trisphosphate Biosynthesis Pathway antibodies  D-myo-inositol (1,4,5)-trisphosphate Biosynthesis Pathway Diagram
 Glycerophospholipid Biosynthetic Pathway antibodies  Glycerophospholipid Biosynthetic Pathway Diagram
 Inositol Phosphate Metabolism Pathway antibodies  Inositol Phosphate Metabolism Pathway Diagram
 Inositol Phosphate Metabolism Pathway antibodies  Inositol Phosphate Metabolism Pathway Diagram
 Inositol Phosphate Metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 Pathway antibodies  Inositol Phosphate Metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 Pathway Diagram
 Inositol Phosphate Metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 Pathway antibodies  Inositol Phosphate Metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 Pathway Diagram

Diseases associated with PI4K2A recombinant protein

 Disease Name  Pubmed Publications
 Abnormalities, Multiple Antibodies  >1 publications with PI4K2A and Abnormalities, Multiple
 Disease Models, Animal Antibodies  >1 publications with PI4K2A and Disease Models, Animal
 Liver Neoplasms Antibodies  >1 publications with PI4K2A and Liver Neoplasms

Organs/Tissues associated with PI4K2A recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >1 publications with PI4K2A and Liver
 Blood Antibodies  >1 publications with PI4K2A and Blood
 Brain Antibodies  >1 publications with PI4K2A and Brain
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