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PI4K2B Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515577

Description: Full-length recombinant human PI4K2Beta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: PI4K2B is a member of the phosphatidylinositol 4-kinase family (PI4K) that phosphorylate… More details

$505 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PI4K2B, Recombinant Protein
Full Product Name
PI4K2B, Active
Product Gene Name
PI4K2B recombinant protein
Product Synonym Gene Name
FLJ11105; PI4KIIB; PIK42B
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
~84 kDa
Sequence Positions
Full length
OMIM
AY065990 mRNA
3D Structure
ModBase 3D Structure for Q8TCG2
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for PI4K2B recombinant protein

Enzymes; Active Lipid Kinases
Applications Tested/Suitable for PI4K2B recombinant protein
Kinase Assay

Testing Data of PI4K2B recombinant protein

PI4K2B Recombinant Protein – Cat# MBS515577

Testing Data #2 of PI4K2B recombinant protein

PI4K2B Recombinant Protein, Testing Data #2 – Cat# MBS515577

Product References and Citations for PI4K2B recombinant protein

1. Balla, A. et.al: Characterization of type II phospha-tidylinositol 4-kinase isoforms reveals association of the enzymes with endosomal vesicular compartments. J. Biol. Chem. 277: 20041-20050, 2002. 2. Griffioen, M. et.al: Identification of phosphatidylinositol 4-kinase type II beta as HLA class II-restricted target in graft versus leukemia reactivity. Proc. Nat. Acad. Sci. 105: 3837-3842, 2008.

Other Notes

Small volumes of PI4K2B recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PI4K2B purchase

MBS515577 is a recombinant protein targeting PI4K2B. Also known as PI4K2B, Active. Host: Sf9 insect cells using baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for PI4K2B. It may not necessarily be applicable to this product.

NCBI GI #
224591400
NCBI GeneID
55300
NCBI Accession #
NM_018323.3
UniProt Primary Accession #
Q8TCG2
UniProt Secondary Accession #
Q9NUW2
UniProt Related Accession #
Q8TCG2
GenBank Nucleotide #
NM_018323
NCBI Official Full Name
Homo sapiens phosphatidylinositol 4-kinase type 2 beta (PI4K2B), transcript variant 1, mRNA
NCBI Official Synonym Full Names
phosphatidylinositol 4-kinase type 2 beta
NCBI Official Symbol
PI4K2B
NCBI Official Synonym Symbols
PIK42B; PI4KIIB
UniProt Protein Name
Phosphatidylinositol 4-kinase type 2-beta
UniProt Synonym Protein Names
Phosphatidylinositol 4-kinase type II-beta; PI4KII-BETA
Protein Family
Phosphatidylinositol 4-kinase
UniProt Gene Name
PI4K2B
UniProt Synonym Gene Names
PI4KII-BETA
NCBI Protein Information
phosphatidylinositol 4-kinase type 2-beta

NCBI Summary for PI4K2B

This gene encodes a member of the type II PI4 kinase protein family. The encoded protein is primarily cytosolic and contributes to overall PI4-kinase activity along with other protein family members. This protein is involved in early T cell activation. [provided by RefSeq, Dec 2016]

UniProt Comments for PI4K2B

PI4K2B: Together with PI4K2A and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. This contribution may be especially significant in plasma membrane, endosomal and Golgi compartments. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells and is likely to be involved in the regulation of vesicular trafficking. Belongs to the PI3/PI4-kinase family. Type II PI4K subfamily.

Protein type: EC 2.7.1.67; Kinase, lipid; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4p15.2

Cellular Component: cytosol; endosome; lysosomal membrane; membrane; plasma membrane; trans-Golgi network

Molecular Function: 1-phosphatidylinositol 4-kinase activity

Biological Process: endosome organization and biogenesis; Golgi organization and biogenesis; phosphatidylinositol biosynthetic process; phosphoinositide phosphorylation
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Research Articles on PI4K2B

1. Here two crystal structures are presented: the structure of human PIK42A and the structure of PIK42B containing a nucleoside analogue.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PI4K2B recombinant protein

 Reference Product  PubMed Publications
 PI4KA recombinant protein  >1 publications with PI4K2B and PI4KA
 PI4KB recombinant protein  >1 publications with PI4K2B and PI4KB

Pathways associated with PI4K2B recombinant protein

 Products by Pathway  Pathway Diagram
 3-phosphoinositide Biosynthesis Pathway antibodies  3-phosphoinositide Biosynthesis Pathway Diagram
 3-phosphoinositide Biosynthesis Pathway antibodies  3-phosphoinositide Biosynthesis Pathway Diagram
 D-myo-inositol (1,4,5)-trisphosphate Biosynthesis Pathway antibodies  D-myo-inositol (1,4,5)-trisphosphate Biosynthesis Pathway Diagram
 D-myo-inositol (1,4,5)-trisphosphate Biosynthesis Pathway antibodies  D-myo-inositol (1,4,5)-trisphosphate Biosynthesis Pathway Diagram
 Inositol Phosphate Metabolism Pathway antibodies  Inositol Phosphate Metabolism Pathway Diagram
 Inositol Phosphate Metabolism Pathway antibodies  Inositol Phosphate Metabolism Pathway Diagram
 Inositol Phosphate Metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 Pathway antibodies  Inositol Phosphate Metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 Pathway Diagram
 Inositol Phosphate Metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 Pathway antibodies  Inositol Phosphate Metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram

Organs/Tissues associated with PI4K2B recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2 publications with PI4K2B and Blood
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