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PIK3C2A Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515578

Description: Recombinant human PIK3C2A (299-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: PIK3C2A is belongs to the phosphoinositide 3-kinase (PI3K) family which play roles in signaling pathways… More details

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Product Overview

Online Datasheet in PDF
Product Name
PIK3C2A, Recombinant Protein
Full Product Name
PIK3C2A, Active
Product Gene Name
PIK3C2A recombinant protein
Product Synonym Gene Name
PI3K-C2 (ALPHA); PI3-K-C2A; DKFZp686L193; CPK; MGC142218
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
155 kDa
Sequence Positions
299-end
OMIM
Y13367 mRNA
3D Structure
ModBase 3D Structure for O00443
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for PIK3C2A recombinant protein

Enzymes; Active Lipid Kinases
Applications Tested/Suitable for PIK3C2A recombinant protein
Kinase Assay

Testing Data of PIK3C2A recombinant protein

PIK3C2A Recombinant Protein – Cat# MBS515578

Testing Data #2 of PIK3C2A recombinant protein

PIK3C2A Recombinant Protein, Testing Data #2 – Cat# MBS515578

Product References and Citations for PIK3C2A recombinant protein

1. Domin, J. et.al: Cloning of a human phosphoinositide 3-kinase with a C2 domain that displays reduced sensitivity to the inhibitor wortmannin. Biochem. J. 326: 139-147, 1997. 2. Molz, L. et.al: Cpk is a novel class of Drosophila PtdIns 3-kinase containing a C2 domain. J. Biol. Chem. 271: 13892-13899, 1996.

Other Notes

Small volumes of PIK3C2A recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PIK3C2A purchase

MBS515578 is a recombinant protein targeting PIK3C2A. Also known as PIK3C2A, Active. Host: Sf9 insect cells using baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for PIK3C2A. It may not necessarily be applicable to this product.

NCBI GI #
1008909361
NCBI GeneID
5286
NCBI Accession #
NM_002645.3
UniProt Primary Accession #
O00443
UniProt Secondary Accession #
Q14CQ9; B0LPH2; B4E2G4
UniProt Related Accession #
O00443
GenBank Nucleotide #
NM_002645
NCBI Official Full Name
Homo sapiens phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha (PIK3C2A), transcript variant 1, mRNA
NCBI Official Synonym Full Names
phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha
NCBI Official Symbol
PIK3C2A
NCBI Official Synonym Symbols
CPK; PI3-K-C2A; PI3K-C2alpha; PI3K-C2-alpha; PI3-K-C2(ALPHA)
UniProt Protein Name
Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha
UniProt Synonym Protein Names
Phosphoinositide 3-kinase-C2-alpha
Protein Family
Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing
UniProt Gene Name
PIK3C2A
UniProt Synonym Gene Names
PI3K-C2-alpha; PtdIns-3-kinase C2 subunit alpha
NCBI Protein Information
phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha

NCBI Summary for PIK3C2A

The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling. [provided by RefSeq, Jul 2008]
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UniProt Comments for PIK3C2A

PIK3C2A: a protein of the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. Its PI3-kinase is not sensitive to nanomolar levels of the inhibitor wortmannin. Activated by insulin and may be involved in integrin-dependent signaling.

Protein type: Autophagy; Carbohydrate Metabolism - inositol phosphate; EC 2.7.1.154; Kinase, lipid; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: cytoplasm; cytosol; membrane; nucleus; phosphoinositide 3-kinase complex; plasma membrane; vesicle

Molecular Function: 1-phosphatidylinositol-3-kinase activity; phosphatidylinositol-4-phosphate 3-kinase activity; phosphoinositide 3-kinase activity

Biological Process: cell migration; clathrin cage assembly; endocytosis; epidermal growth factor receptor signaling pathway; exocytosis; insulin receptor signaling pathway; phosphatidylinositol biosynthetic process; phosphoinositide 3-kinase cascade; platelet-derived growth factor receptor signaling pathway; vascular smooth muscle contraction
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Research Articles on PIK3C2A

1. miR-518a-5p was downregulated in imatinib-resistant gastrointestinal stromal tumors (GISTs), and the expression of miR-518a-5p was confirmed with good concordance between real-time PCR and miRNA microarray results. Luciferase reporter assays indicated that miR-518a-5p bound to the PIK3C2A 3'UTR.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PIK3C2A recombinant protein

 Reference Product  PubMed Publications
 PIKFYVE recombinant protein  >1 publications with PIK3C2A and PIKFYVE

Pathways associated with PIK3C2A recombinant protein

 Products by Pathway  Pathway Diagram
 3-phosphoinositide Biosynthesis Pathway antibodies  3-phosphoinositide Biosynthesis Pathway Diagram
 3-phosphoinositide Biosynthesis Pathway antibodies  3-phosphoinositide Biosynthesis Pathway Diagram
 Angiopoietin Like Protein 8 Regulatory Pathway antibodies  Angiopoietin Like Protein 8 Regulatory Pathway Diagram
 Clathrin Derived Vesicle Budding Pathway antibodies  Clathrin Derived Vesicle Budding Pathway Diagram
 Clathrin-mediated Endocytosis Pathway antibodies  Clathrin-mediated Endocytosis Pathway Diagram
 DNA Damage Response (only ATM Dependent) Pathway antibodies  DNA Damage Response (only ATM Dependent) Pathway Diagram
 Golgi Associated Vesicle Biogenesis Pathway antibodies  Golgi Associated Vesicle Biogenesis Pathway Diagram
 Inositol Phosphate Metabolism Pathway antibodies  Inositol Phosphate Metabolism Pathway Diagram
 Inositol Phosphate Metabolism Pathway antibodies  Inositol Phosphate Metabolism Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram

Diseases associated with PIK3C2A recombinant protein

 Disease Name  Pubmed Publications
 Prostatic Neoplasms Antibodies  >2 publications with PIK3C2A and Prostatic Neoplasms
 Carcinoma, Hepatocellular Antibodies  >2 publications with PIK3C2A and Carcinoma, Hepatocellular
 Cardiovascular Diseases Antibodies  >2 publications with PIK3C2A and Cardiovascular Diseases
 Learning Disorders Antibodies  >1 publications with PIK3C2A and Learning Disorders
 Neoplasm Metastasis Antibodies  >1 publications with PIK3C2A and Neoplasm Metastasis
 Lung Neoplasms Antibodies  >1 publications with PIK3C2A and Lung Neoplasms
 Brain Diseases Antibodies  >1 publications with PIK3C2A and Brain Diseases
 Breast Neoplasms Antibodies  >1 publications with PIK3C2A and Breast Neoplasms
 Liver Diseases Antibodies  >1 publications with PIK3C2A and Liver Diseases
 Liver Neoplasms Antibodies  >1 publications with PIK3C2A and Liver Neoplasms

Organs/Tissues associated with PIK3C2A recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >7 publications with PIK3C2A and Blood
 Vascular Antibodies  >5 publications with PIK3C2A and Vascular
 Muscle Antibodies  >4 publications with PIK3C2A and Muscle
 Lung Antibodies  >3 publications with PIK3C2A and Lung
 Brain Antibodies  >3 publications with PIK3C2A and Brain
 Prostate Antibodies  >2 publications with PIK3C2A and Prostate
 Intestine Antibodies  >2 publications with PIK3C2A and Intestine
 Pancreas Antibodies  >2 publications with PIK3C2A and Pancreas
 Bone Antibodies  >2 publications with PIK3C2A and Bone
 Heart Antibodies  >2 publications with PIK3C2A and Heart
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