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PI4KB Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515596

Description: Full-length recombinant human PI4KB was co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: PI4KB is a member of the phosphatidylinositol 4-kinase family (PI4K) and is the first committed step in the… More details

$505 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PI4KB, Recombinant Protein
Full Product Name
PI4KB, Active
Product Gene Name
PI4KB recombinant protein
Product Synonym Gene Name
PI4K-BETA; pi4K92; PI4Kbeta; PI4KIIIbeta
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
~120 kDa
Sequence Positions
Full length
OMIM
AJ011121 mRNA
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for PI4KB recombinant protein

Enzymes; Active Lipid Kinases
Applications Tested/Suitable for PI4KB recombinant protein
Kinase Assay

Testing Data of PI4KB recombinant protein

PI4KB Recombinant Protein – Cat# MBS515596

Testing Data #2 of PI4KB recombinant protein

PI4KB Recombinant Protein, Testing Data #2 – Cat# MBS515596

Product References and Citations for PI4KB recombinant protein

1. Balla, A. et.al: Characterization of type II phospha-tidylinositol 4-kinase isoforms reveals association of the enzymes with endosomal vesicular compartments. J. Biol. Chem. 277: 20041-20050, 2002. 2. Griffioen, M. et.al: Identification of phosphatidylinositol 4-kinase type II beta as HLA class II-restricted target in graft versus leukemia reactivity. Proc. Nat. Acad. Sci. 105: 3837-3842, 2008.

Other Notes

Small volumes of PI4KB recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PI4KB purchase

MBS515596 is a recombinant protein targeting PI4KB. Also known as PI4KB, Active. Host: Sf9 insect cells using baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for PI4KB. It may not necessarily be applicable to this product.

NCBI GI #
33875124
NCBI GeneID
5298
NCBI Accession #
BC000029
UniProt Secondary Accession #
O15096; P78405; Q5VWB9; Q5VWC0; Q5VWC1; Q9BWR6; B4DGI2
UniProt Related Accession #
Q9UBF8
NCBI Official Full Name
Homo sapiens phosphatidylinositol 4-kinase, catalytic, beta, mRNA
NCBI Official Synonym Full Names
phosphatidylinositol 4-kinase beta
NCBI Official Symbol
PI4KB
NCBI Official Synonym Symbols
NPIK; PI4K92; PIK4CB; PI4KBETA; PI4K-BETA; PI4KIIIBETA
UniProt Protein Name
Phosphatidylinositol 4-kinase beta
UniProt Synonym Protein Names
NPIK; PI4K92
Protein Family
Phosphatidylinositol 4-kinase
UniProt Gene Name
PI4KB
UniProt Synonym Gene Names
PIK4CB; PI4K-beta; PI4Kbeta; PtdIns 4-kinase beta
NCBI Protein Information
phosphatidylinositol 4-kinase beta

UniProt Comments for PI4KB

PIK4CB: a member of the PI3/PI4 kinase family. Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol- 1,4,5,-trisphosphate. Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5-biphosphate (PIP2) on the Golgi complex. May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Three splice-variant isoforms have been described.

Protein type: Carbohydrate Metabolism - inositol phosphate; EC 2.7.1.67; Kinase, lipid; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cytoplasm; cytosol; endosome; Golgi membrane; membrane

Molecular Function: 1-phosphatidylinositol 4-kinase activity; protein binding

Biological Process: phosphatidylinositol biosynthetic process; phosphoinositide-mediated signaling; receptor-mediated endocytosis; signal transduction
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Research Articles on PI4KB

1. The results showed that, in contrast to the enteroviruses and the cardioviruses, foot-and-mouth disease virus replication does not require PI4KIII (PI4KIIIalpha and PI4KIIIbeta), and phosphatidylinositol 4-phosphate levels do not increase in foot-and-mouth disease virus-infected cells and phosphatidylinositol 4-phosphate is not seen at replication organelles.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with PI4KB recombinant protein

 Products by Pathway  Pathway Diagram
 3-phosphoinositide Biosynthesis Pathway antibodies  3-phosphoinositide Biosynthesis Pathway Diagram
 3-phosphoinositide Biosynthesis Pathway antibodies  3-phosphoinositide Biosynthesis Pathway Diagram
 D-myo-inositol (1,4,5)-trisphosphate Biosynthesis Pathway antibodies  D-myo-inositol (1,4,5)-trisphosphate Biosynthesis Pathway Diagram
 D-myo-inositol (1,4,5)-trisphosphate Biosynthesis Pathway antibodies  D-myo-inositol (1,4,5)-trisphosphate Biosynthesis Pathway Diagram
 Inositol Phosphate Metabolism Pathway antibodies  Inositol Phosphate Metabolism Pathway Diagram
 Inositol Phosphate Metabolism Pathway antibodies  Inositol Phosphate Metabolism Pathway Diagram
 Inositol Phosphate Metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 Pathway antibodies  Inositol Phosphate Metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 Pathway Diagram
 Inositol Phosphate Metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 Pathway antibodies  Inositol Phosphate Metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 Pathway Diagram
 Integrins In Angiogenesis Pathway antibodies  Integrins In Angiogenesis Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram

Diseases associated with PI4KB recombinant protein

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >2 publications with PI4KB and Nervous System Diseases
 Liver Diseases Antibodies  >1 publications with PI4KB and Liver Diseases

Organs/Tissues associated with PI4KB recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >1 publications with PI4KB and Bone
 Liver Antibodies  >1 publications with PI4KB and Liver
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