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PNMT Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515599

Description: Full-length recombinant human PNMT was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: Phenylethanolamine N-methyltransferase (PNMT) catalyzes the biosynthesis of adrenaline though methylation of… More details

$505 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PNMT, Recombinant Protein
Full Product Name
PNMT, Active
Product Gene Name
PNMT recombinant protein
Product Synonym Gene Name
PENT; PNMTase
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
57 kDa
Sequence Positions
Full length
OMIM
J03727 mRNA
3D Structure
ModBase 3D Structure for P11086
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for PNMT recombinant protein

Enzymes; Active Methyltransferases
Applications Tested/Suitable for PNMT recombinant protein
Methyltransferase Assay

Testing Data of PNMT recombinant protein

PNMT Recombinant Protein – Cat# MBS515599

Testing Data #2 of PNMT recombinant protein

PNMT Recombinant Protein, Testing Data #2 – Cat# MBS515599

Product References and Citations for PNMT recombinant protein

1. Kaneda, N., et al. Molecular cloning of cDNA and chromosomal assignment of the gene for human phenylethanolamine N-methyltransferase, the enzyme for epinephrine biosynthesis. J Biol Chem. 263 (16):7672-7, 1988. 2. Mann, M.B., et al. Phenylethanolamine N-methyltransferase (PNMT) gene and early-onset Alzheimer disease. Am J Med Genet. 105 (4):312-6, 2001. 3. Gearhart, D.A., et al. Phenylethanolamine N-methyltransferase has beta-carboline 2N-methyltransferase activity: hypothetical relevance to Parkinson's disease. Neurochem Int. 40 (7):611-20, 2002. 4. Mann, M.B., et al. Association between the phenylethanolamine N-methyltransferase gene and multiple sclerosis. J Neuroimmunol. 124 (1-2):101-5, 2002. 5. Evinger, M.J., et al. Nicotine stimulates expression of the PNMT gene through a novel promoter sequence. J Mol Neurosci. 26 (1):39-55, 2005.

Other Notes

Small volumes of PNMT recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PNMT purchase

MBS515599 is a recombinant protein targeting PNMT. Also known as PNMT, Active. Host: Sf9 insect cells using baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for PNMT. It may not necessarily be applicable to this product.

NCBI GI #
425703051
NCBI GeneID
5409
NCBI Accession #
NM_002686.4
UniProt Primary Accession #
P11086
UniProt Related Accession #
P11086
GenBank Nucleotide #
NM_002686
NCBI Official Full Name
Homo sapiens phenylethanolamine N-methyltransferase (PNMT), transcript variant 1, mRNA
NCBI Official Synonym Full Names
phenylethanolamine N-methyltransferase
NCBI Official Symbol
PNMT
NCBI Official Synonym Symbols
PENT; PNMTase
UniProt Protein Name
Phenylethanolamine N-methyltransferase
UniProt Synonym Protein Names
Noradrenaline N-methyltransferase
Protein Family
Phenylethanolamine N-methyltransferase
UniProt Gene Name
PNMT
UniProt Synonym Gene Names
PENT; PNMTase
NCBI Protein Information
phenylethanolamine N-methyltransferase

NCBI Summary for PNMT

The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012]
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UniProt Comments for PNMT

PNMT: Converts noradrenaline to adrenaline. Belongs to the NNMT/PNMT/TEMT family.

Protein type: Amino Acid Metabolism - tyrosine; EC 2.1.1.28; Methyltransferase

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: cytosol

Molecular Function: phenylethanolamine N-methyltransferase activity

Biological Process: catecholamine biosynthetic process

Disease: Hypertension, Essential
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Research Articles on PNMT

1. initial velocity studies on human PNMT in the absence and presence of product and dead end inhibitors.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PNMT recombinant protein

 Reference Product  PubMed Publications
 DBH recombinant protein  >191 publications with PNMT and DBH
 COMT recombinant protein  >29 publications with PNMT and COMT
 TCAP recombinant protein  >9 publications with PNMT and TCAP
 MAOA recombinant protein  >4 publications with PNMT and MAOA
 MAOB recombinant protein  >1 publications with PNMT and MAOB

Pathways associated with PNMT recombinant protein

 Products by Pathway  Pathway Diagram
 Amine-derived Hormones Pathway antibodies  Amine-derived Hormones Pathway Diagram
 Amino Acid Metabolism Pathway antibodies  Amino Acid Metabolism Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Catecholamine Biosynthesis Pathway antibodies  Catecholamine Biosynthesis Pathway Diagram
 Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway antibodies  Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway Diagram
 Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway antibodies  Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Methylation Pathways antibodies  Methylation Pathways Diagram

Diseases associated with PNMT recombinant protein

 Disease Name  Pubmed Publications
 Hypertension Antibodies  >74 publications with PNMT and Hypertension
 Disease Models, Animal Antibodies  >30 publications with PNMT and Disease Models, Animal
 Hypotension Antibodies  >14 publications with PNMT and Hypotension
 Pain Antibodies  >12 publications with PNMT and Pain
 Heart Diseases Antibodies  >12 publications with PNMT and Heart Diseases
 Bradycardia Antibodies  >9 publications with PNMT and Bradycardia
 Inflammation Antibodies  >8 publications with PNMT and Inflammation
 Heart Failure Antibodies  >8 publications with PNMT and Heart Failure
 Kidney Diseases Antibodies  >8 publications with PNMT and Kidney Diseases
 Movement Disorders Antibodies  >7 publications with PNMT and Movement Disorders

Organs/Tissues associated with PNMT recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >456 publications with PNMT and Brain
 Adrenal Gland Antibodies  >343 publications with PNMT and Adrenal Gland
 Eye Antibodies  >26 publications with PNMT and Eye
 Thyroid Antibodies  >10 publications with PNMT and Thyroid
 Placenta Antibodies  >2 publications with PNMT and Placenta
 Uterus Antibodies  >2 publications with PNMT and Uterus
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