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PDE1A Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515627

Description: Recombinant full-length human PDE1A was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.Scientific Background: PDE1A is a member of the cyclic nucleotide phosphodiesterase family that is stimulated by Ca2+-calmodulin.… More details

$505 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PDE1A, Active Protein
Full Product Name
PDE1A, Active
Product Gene Name
PDE1A active protein
Product Synonym Gene Name
HCAM1; HSPDE1A; MGC26303
Isotype
Sf9 cells
Form/Format
Recombinant protein stored in 50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Purity/Purification
>80%
Concentration
0.1 ug/ul (lot specific)
Specificity
Full length protein expressed in Sf9 cells
Preparation and Storage
Ships on dry ice. Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
~64 kDa
OMIM
U40370 mRNA
3D Structure
ModBase 3D Structure for P54750
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Specific Activity
10,500 nmol/min/mg
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for PDE1A active protein

Enzymes; Active Phosphodiesterases

Specific Activity of PDE1A active protein

The specific activity of PDE1A was determined to be 10,500 nmol/min/mg as per activity assay protocol
PDE1A Active Protein – Cat# MBS515627

Puity of PDE1A active protein

The purity was determined to be >80% by densitometry. Approx. MW 64kDa.
PDE1A Active Protein, Puity – Cat# MBS515627

Product References and Citations for PDE1A active protein

1. Lefi¨vre L, et al: Presence of cyclic nucleotide phosphodiesterases PDE1A, existing as a stable complex with calmodulin, and PDE3A in human spermatozoa. Biol Reprod. 2002 Aug;67 (2):423-30. 2. Nagel D J, et al: Role of nuclear Ca2+/calmodulin-stimulated phosphodiesterase 1A in vascular smooth muscle cell growth and survival. Circ Res. 2006 Mar 31;98 (6):777-84.

Other Notes

Small volumes of PDE1A active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDE1A purchase

MBS515627 is an active protein targeting PDE1A. Also known as PDE1A, Active.

Additional Details

NCBI/Uniprot data below describe general gene information for PDE1A. It may not necessarily be applicable to this product.

NCBI GI #
385198066
NCBI GeneID
5136
NCBI Accession #
NM_005019.4
UniProt Primary Accession #
P54750
UniProt Secondary Accession #
Q86VZ0; Q9C0K8; Q9C0K9; Q9C0L0; Q9C0L1; Q9C0L2; Q9C0L3; Q9C0L4; Q9UFX3; D3DPG5
UniProt Related Accession #
P54750
GenBank Nucleotide #
NM_005019
NCBI Official Full Name
Homo sapiens phosphodiesterase 1A (PDE1A), transcript variant 1, mRNA
NCBI Official Synonym Full Names
phosphodiesterase 1A
NCBI Official Symbol
PDE1A
NCBI Official Synonym Symbols
HCAM1; HCAM-1; HSPDE1A; CAM-PDE-1A
UniProt Protein Name
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A
UniProt Synonym Protein Names
61 kDa Cam-PDE; hCam-1
Protein Family
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase
UniProt Gene Name
PDE1A
UniProt Synonym Gene Names
Cam-PDE 1A
NCBI Protein Information
calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A

NCBI Summary for PDE1A

Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1; MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al., 2001 [PubMed 11342109]; Fidock et al., 2002 [PubMed 11747989]).[supplied by OMIM, Oct 2009]
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UniProt Comments for PDE1A

PDE1A: Cyclic nucleotide phosphodiesterase with a dual- specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a higher affinity for cGMP than for cAMP. Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.17; Nucleotide Metabolism - purine; Phosphodiesterase

Chromosomal Location of Human Ortholog: 2q32.1

Cellular Component: cytosol

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; 3',5'-cyclic-GMP phosphodiesterase activity; calmodulin-dependent cyclic-nucleotide phosphodiesterase activity
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Research Articles on PDE1A

1. Report significant associations of PDE1A single nucleotide polymorphisms with diastolic blood pressure and carotid intima-media thickness.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with PDE1A active protein

 Products by Pathway  Pathway Diagram
 Ca-dependent Events Pathway antibodies  Ca-dependent Events Pathway Diagram
 CaM Pathway antibodies  CaM Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calmodulin Induced Events Pathway antibodies  Calmodulin Induced Events Pathway Diagram
 Cam-PDE 1 Activation Pathway antibodies  Cam-PDE 1 Activation Pathway Diagram
 DAG And IP3 Signaling Pathway antibodies  DAG And IP3 Signaling Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram

Diseases associated with PDE1A active protein

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >16 publications with PDE1A and Cardiovascular Diseases
 Hypertension Antibodies  >14 publications with PDE1A and Hypertension
 Hypertrophy Antibodies  >6 publications with PDE1A and Hypertrophy
 Cardiomegaly Antibodies  >5 publications with PDE1A and Cardiomegaly
 Inflammation Antibodies  >4 publications with PDE1A and Inflammation
 Disease Models, Animal Antibodies  >3 publications with PDE1A and Disease Models, Animal
 Fibrosis Antibodies  >2 publications with PDE1A and Fibrosis
 Kidney Diseases Antibodies  >2 publications with PDE1A and Kidney Diseases
 Immune System Diseases Antibodies  >2 publications with PDE1A and Immune System Diseases
 Neurobehavioral Manifestations Antibodies  >2 publications with PDE1A and Neurobehavioral Manifestations

Organs/Tissues associated with PDE1A active protein

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >30 publications with PDE1A and Muscle
 Brain Antibodies  >13 publications with PDE1A and Brain
 Lung Antibodies  >11 publications with PDE1A and Lung
 Kidney Antibodies  >7 publications with PDE1A and Kidney
 Thyroid Antibodies  >4 publications with PDE1A and Thyroid
 Testis Antibodies  >4 publications with PDE1A and Testis
 Eye Antibodies  >3 publications with PDE1A and Eye
 Embryonic Tissue Antibodies  >2 publications with PDE1A and Embryonic Tissue
 Ganglia Antibodies  >2 publications with PDE1A and Ganglia
 Trachea Antibodies  >1 publications with PDE1A and Trachea
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