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PDE4D Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515633

Description: Recombinant human PDE4D (225-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: PDE4D is a member of the phosphodiesterase (PDE) family that is cAMP-specific and has homology to the 'dunce'… More details

$505 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PDE4D, Recombinant Protein
Full Product Name
PDE4D, Active
Product Gene Name
Product Synonym Gene Name
DPDE3; STRK1; HSPDE4D; PDE4DN2
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCI, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Purity/Purification
>85%
Concentration
0.1ug/ul (lot specific)
Preparation and Storage
Store product at –70°C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Ships on dry ice.
Molecular Weight
~82 kDa
Sequence Positions
225-end
OMIM
L20970 mRNA
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Applications Tested/Suitable for PDE4D recombinant protein
PDE Assay

Testing Data of PDE4D recombinant protein

the specific activity of PDE4D was determined to be 1,250 nmol/min/mg as per activity assay protocol.
PDE4D Recombinant Protein – Cat# MBS515633

Testing Data #2 of PDE4D recombinant protein

PDE4D Recombinant Protein, Testing Data #2 – Cat# MBS515633

Product References and Citations for PDE4D recombinant protein

1. Miro X, et al: Phosphodiesterases 4D and 7A splice variants in the response of HUVEC cells to TNF-alpha. Biochem. Biophys. Res. Commun. 274: 415-421, 2000. 2. Jin S-L C, et al: Impaired growth and fertility of cAMP-specific phosphodiesterase PDE4D-deficient mice. Proc. Nat. Acad. Sci. 96: 11998-12003, 1999.

Other Notes

Small volumes of PDE4D recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDE4D purchase

MBS515633 is a recombinant protein targeting PDE4D. Also known as PDE4D, Active. Host: Sf9 insect cells using baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for PDE4D. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens phosphodiesterase 4D (PDE4D), transcript variant 2, mRNA
NCBI Official Synonym Full Names
phosphodiesterase 4D
NCBI Official Symbol
PDE4D
NCBI Official Synonym Symbols
DPDE3; PDE43; STRK1; ACRDYS2; HSPDE4D; PDE4DN2
UniProt Protein Name
cAMP-specific 3',5'-cyclic phosphodiesterase 4D
UniProt Synonym Protein Names
DPDE3; PDE43
UniProt Gene Name
PDE4D
UniProt Synonym Gene Names
DPDE3
NCBI Protein Information
cAMP-specific 3',5'-cyclic phosphodiesterase 4D

NCBI Summary for PDE4D

This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.[provided by RefSeq, Sep 2009]
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UniProt Comments for PDE4D

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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