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PDE4A Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515634

Description: Recombinant human PDE4A (332-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: PDE4A is a member of the phosphodiesterase family of proteins that play a critical role in regulating… More details

$505 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PDE4A, Active Protein
Full Product Name
PDE4A, Active
Product Gene Name
PDE4A active protein
Product Synonym Gene Name
PDE4; DPDE2; PDE46
Host
Sf9 cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Purity/Purification
>95%
Concentration
0.1 ug/ml (lot specific)
Preparation and Storage
Shipping: Dry Ice
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
~110 kDa
OMIM
L20965 mRNA
3D Structure
ModBase 3D Structure for P27815
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Specific Activity
1082 nmol/min/mg
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for PDE4A active protein

Enzymes; Active Phosphodiesterases

Testing Data of PDE4A active protein

The specific activity of PDE4A was determined to be 1082 nmol/min/mg as per activity assay protocol.
PDE4A Active Protein – Cat# MBS515634

Testing Data #2 of PDE4A active protein

The purity was determined to be > 95% by densitometry.
PDE4A Active Protein, Testing Data #2 – Cat# MBS515634

Product References and Citations for PDE4A active protein

1. Goldhoff P, et al: Targeted inhibition of cyclic AMP phosphodiesterase-4 promotes brain tumor regression. Clin Cancer Res. 2008 Dec 1;14 (23):7717-25. 2. Fatemi S H, et al: Phosphodiesterase-4A expression is reduced in cerebella of patients with bipolar disorder. Psychiatr Genet. 2008 Dec;18 (6):282-8.

Other Notes

Small volumes of PDE4A active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDE4A purchase

MBS515634 is an active protein targeting PDE4A. Also known as PDE4A, Active. Host: Sf9 cells.

Additional Details

NCBI/Uniprot data below describe general gene information for PDE4A. It may not necessarily be applicable to this product.

NCBI GI #
162329607
NCBI GeneID
5141
NCBI Accession #
NM_001111307.1
UniProt Primary Accession #
P27815
UniProt Secondary Accession #
O75522; O76092; Q16255; Q16691; Q5DM53; Q6PMT2; Q8IVA7; Q8WUQ3; Q9H3H2
UniProt Related Accession #
P27815
GenBank Nucleotide #
NM_001111307
NCBI Official Full Name
Homo sapiens phosphodiesterase 4A (PDE4A), transcript variant 1, mRNA
NCBI Official Synonym Full Names
phosphodiesterase 4A
NCBI Official Symbol
PDE4A
NCBI Official Synonym Symbols
PDE4; DPDE2; PDE46
UniProt Protein Name
cAMP-specific 3',5'-cyclic phosphodiesterase 4A
UniProt Synonym Protein Names
DPDE2; PDE46
Protein Family
cAMP-specific 3',5'-cyclic phosphodiesterase
UniProt Gene Name
PDE4A
UniProt Synonym Gene Names
DPDE2
NCBI Protein Information
cAMP-specific 3',5'-cyclic phosphodiesterase 4A

NCBI Summary for PDE4A

The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]
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UniProt Comments for PDE4A

PDE4A: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. Isoform 1 interacts with LYN. Isoform 2 and isoform 6 interact weakly with LYN. Isoform 1, isoform 2 and isoform 6 interact with ARRB2. Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum. Isoform 1, isoform 2, isoform 6 and isoform 7 are inhibited by rolipram and cilomilast. Isoform 1, isoform 2 and isoform 6 are inhibited by 4-[(3-butoxy-4-methoxyphenyl)-methyl]- 2-imidazolidinone (Ro 20-1724), roflumilast and denbufylline. Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.53; Nucleotide Metabolism - purine; Phosphodiesterase

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: cytosol; membrane; nucleoplasm; perinuclear region of cytoplasm; plasma membrane

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; cAMP binding; protein binding

Biological Process: cAMP catabolic process
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Research Articles on PDE4A

1. A multifunctional docking site on the catalytic unit of PDE4 that is utilized by multiple interaction partners has been identified.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PDE4A active protein

 Reference Product  PubMed Publications
 ADCY10 active protein  >2 publications with PDE4A and ADCY10
 ADCY9 active protein  >2 publications with PDE4A and ADCY9
 ADCY7 active protein  >2 publications with PDE4A and ADCY7
 ADCY3 active protein  >1 publications with PDE4A and ADCY3

Pathways associated with PDE4A active protein

 Products by Pathway  Pathway Diagram
 DARPP-32 Events Pathway antibodies  DARPP-32 Events Pathway Diagram
 G Protein Signaling Pathways antibodies  G Protein Signaling Pathways Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 Morphine Addiction Pathway antibodies  Morphine Addiction Pathway Diagram
 Morphine Addiction Pathway antibodies  Morphine Addiction Pathway Diagram
 Opioid Signalling Pathway antibodies  Opioid Signalling Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram

Diseases associated with PDE4A active protein

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >25 publications with PDE4A and Inflammation
 Necrosis Antibodies  >22 publications with PDE4A and Necrosis
 Disease Models, Animal Antibodies  >16 publications with PDE4A and Disease Models, Animal
 Memory Disorders Antibodies  >8 publications with PDE4A and Memory Disorders
 Cardiovascular Diseases Antibodies  >8 publications with PDE4A and Cardiovascular Diseases
 Cognition Disorders Antibodies  >8 publications with PDE4A and Cognition Disorders
 Heart Diseases Antibodies  >6 publications with PDE4A and Heart Diseases
 Hypertension Antibodies  >6 publications with PDE4A and Hypertension
 Learning Disorders Antibodies  >5 publications with PDE4A and Learning Disorders
 Fibrosis Antibodies  >5 publications with PDE4A and Fibrosis

Organs/Tissues associated with PDE4A active protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >64 publications with PDE4A and Brain
 Blood Antibodies  >46 publications with PDE4A and Blood
 Lung Antibodies  >25 publications with PDE4A and Lung
 Muscle Antibodies  >24 publications with PDE4A and Muscle
 Testis Antibodies  >14 publications with PDE4A and Testis
 Kidney Antibodies  >11 publications with PDE4A and Kidney
 Bone Antibodies  >10 publications with PDE4A and Bone
 Liver Antibodies  >5 publications with PDE4A and Liver
 Embryonic Tissue Antibodies  >3 publications with PDE4A and Embryonic Tissue
 Spleen Antibodies  >2 publications with PDE4A and Spleen
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