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SMYD2 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515673

Description: Recombinant full-length human SMYD2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: SMYD2 (also known as SET and MYND domain-containing protein 2) is a member of the SMYD family of protein… More details

$505 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SMYD2, Recombinant Protein
Full Product Name
SMYD2 (KMT3C), Active
Product Gene Name
SMYD2 recombinant protein
Product Synonym Gene Name
KMT3C; HSKM-B; ZMYND14
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
70 kDa
Sequence Positions
Full length
OMIM
AF226053 mRNA
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

Product Categories/Family for SMYD2 recombinant protein

Enzymes; Active Methyltransferases
Applications Tested/Suitable for SMYD2 recombinant protein
Methyltransferase Assay

Testing Data of SMYD2 recombinant protein

SMYD2 Recombinant Protein – Cat# MBS515673

Testing Data #2 of SMYD2 recombinant protein

SMYD2 Recombinant Protein, Testing Data #2 – Cat# MBS515673

Product References and Citations for SMYD2 recombinant protein

1. Brown, M. A. et.al: Identification and characterization of Smyd2: a split SET/MYND domain-containing histone H3 lysine 36-specific methyltransferase that interacts with the Sin3 histone deacetylase complex. Molec. Cancer 5: 26, 2006. 2.Huang, J. et.al: Repression of p53 activity by Smyd2-mediated methylation. Nature 444: 629-632, 2006.

Other Notes

Small volumes of SMYD2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SMYD2 purchase

MBS515673 is a recombinant protein targeting SMYD2. Also known as SMYD2 (KMT3C), Active. Host: Sf9 insect cells using baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for SMYD2. It may not necessarily be applicable to this product.

NCBI GI #
29387398
NCBI GeneID
56950
NCBI Accession #
BC049367
UniProt Secondary Accession #
Q4V765; Q5VSH9; Q96AI4; B2R9P9; I6L9H7
UniProt Related Accession #
Q9NRG4
NCBI Official Full Name
Homo sapiens SET and MYND domain containing 2, mRNA
NCBI Official Synonym Full Names
SET and MYND domain containing 2
NCBI Official Symbol
SMYD2
NCBI Official Synonym Symbols
KMT3C; HSKM-B; ZMYND14
UniProt Protein Name
N-lysine methyltransferase SMYD2
UniProt Synonym Protein Names
HSKM-B; Histone methyltransferase SMYD2 (EC:2.1.1.43); Lysine N-methyltransferase 3C; SET and MYND domain-containing protein 2
Protein Family
N-lysine methyltransferase
UniProt Gene Name
SMYD2
UniProt Synonym Gene Names
KMT3C
NCBI Protein Information
N-lysine methyltransferase SMYD2

NCBI Summary for SMYD2

SET domain-containing proteins, such as SMYD2, catalyze lysine methylation (Brown et al., 2006 [PubMed 16805913]).[supplied by OMIM, Nov 2008]

UniProt Comments for SMYD2

SMYD2: Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Has also methyltransferase activity toward non-histone proteins such as p53/TP53 and RB1. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates 'Lys-860' of RB1/RB. Interacts with RNA polymerase II and HELZ. Interacts with SIN3A and HDAC1. Interacts (via MYND-type zinc finger) with EPB41L3. Interacts (via SET domain) with p53/TP53. Interacts with RB1 and HSP90AA1. Expression is repressed by CEBPA.

Protein type: EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 1q32.3

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: histone lysine N-methyltransferase activity (H3-K36 specific); histone-lysine N-methyltransferase activity; lysine N-methyltransferase activity; p53 binding; protein binding; protein-lysine N-methyltransferase activity

Biological Process: negative regulation of cell proliferation; negative regulation of transcription from RNA polymerase II promoter; peptidyl-lysine di-methylation; peptidyl-lysine mono-methylation; regulation of DNA damage response, signal transduction by p53 class mediator
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Research Articles on SMYD2

1. High expression of SMYD2 is associated with chronic lymphocytic leukemia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SMYD2 recombinant protein

 Reference Product  PubMed Publications
 TP53 recombinant protein  >4 publications with SMYD2 and TP53
 RB1 recombinant protein  >1 publications with SMYD2 and RB1

Pathways associated with SMYD2 recombinant protein

 Products by Pathway  Pathway Diagram
 P53 Pathway antibodies  P53 Pathway Diagram

Diseases associated with SMYD2 recombinant protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >8 publications with SMYD2 and Neoplasms
 Breast Neoplasms Antibodies  >2 publications with SMYD2 and Breast Neoplasms
 Nervous System Diseases Antibodies  >1 publications with SMYD2 and Nervous System Diseases
 Central Nervous System Diseases Antibodies  >1 publications with SMYD2 and Central Nervous System Diseases
 Embryo Loss Antibodies  >1 publications with SMYD2 and Embryo Loss

Organs/Tissues associated with SMYD2 recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >6 publications with SMYD2 and Muscle
 Heart Antibodies  >5 publications with SMYD2 and Heart
 Embryonic Tissue Antibodies  >3 publications with SMYD2 and Embryonic Tissue
 Brain Antibodies  >3 publications with SMYD2 and Brain
 Bladder Antibodies  >1 publications with SMYD2 and Bladder
 Nerve Antibodies  >1 publications with SMYD2 and Nerve
 Lung Antibodies  >1 publications with SMYD2 and Lung
 Lymph Node Antibodies  >1 publications with SMYD2 and Lymph Node
 Mammary Gland Antibodies  >1 publications with SMYD2 and Mammary Gland
 Lymph Antibodies  >1 publications with SMYD2 and Lymph
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