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SETD6 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS515683

Description: Recombinant full-length human SETD6 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.Scientific Background: SET domain-containing protein 6 (SETD6) is a mono-methyltransferase that has been shown to methylate RelA of… More details

$505 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SETD6, Recombinant Protein
Full Product Name
SETD6, Active
Product Gene Name
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight
75 kDa
Sequence Positions
Full length
OMIM
AK024801 mRNA
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Applications Tested/Suitable for SETD6 recombinant protein
Methyltransferase Assay

Testing Data #2 of SETD6 recombinant protein

SETD6 Recombinant Protein, Testing Data #2 – Cat# MBS515683

Product References and Citations for SETD6 recombinant protein

1. Levy D, et al: Lysine methylation of the NF-kappaB subunit RelA by SETD6 couples activity of the histone methyl-transferase GLP at chromatin to tonic repression of NF-kappaB signaling. Nat Immunol. 2011 12 (1):29-36. 2. Binda O, et al: SETD6 monomethylates H2AZ on lysine 7 and is required for the maintenance of embryonic stem cell self-renewal. Epigenetics. 2013 8 (2):177-83.

Other Notes

Small volumes of SETD6 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SETD6 purchase

MBS515683 is a recombinant protein targeting SETD6. Also known as SETD6, Active. Host: Sf9 insect cells using baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for SETD6. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens SET domain containing 6 (SETD6), transcript variant 1, mRNA
NCBI Official Synonym Full Names
SET domain containing 6
NCBI Official Symbol
SETD6
UniProt Protein Name
N-lysine methyltransferase SETD6
UniProt Synonym Protein Names
SET domain-containing protein 6
UniProt Gene Name
SETD6
NCBI Protein Information
N-lysine methyltransferase SETD6

NCBI Summary for SETD6

This gene encodes a methyltransferase that adds a methyl group to the histone H2AZ, which is involved in nuclear receptor-dependent transcription. The protein also interacts with several endogenous proteins which are involved in nuclear hormone receptor signaling. A related pseudogene is located on chromosome 2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
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UniProt Comments for SETD6

SETD6: Protein-lysine N-methyltransferase that specifically monomethylates 'Lys-310' of the RELA subunit of NF-kappa-B complex, leading to down-regulate NF-kappa-B transcription factor activity. Belongs to the SETD6 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.-; Methyltransferase; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: cytosol; nucleoplasm; nucleus

Molecular Function: NF-kappaB binding; protein binding; protein-lysine N-methyltransferase activity

Biological Process: inhibition of NF-kappaB transcription factor; peptidyl-lysine mono-methylation; regulation of inflammatory response; stem cell differentiation; stem cell maintenance
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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