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KLRD1 Antibody – Rat Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS673581

MBS673581 is an antibody targeting KLRD1. Also known as Rat Anti-Mouse CD94-UNLB. Reactive in Mouse. Host: Rat. More details

$335 / 0.5 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD94 (KLRD1), Monoclonal Antibody
Full Product Name
Rat Anti-Mouse CD94-UNLB
Product Gene Name
Product Synonym Names
Rat Anti-Mouse CD94 - Purified, Unlabeled Antibody; Rat Anti-Mouse CD94
Clonality
Monoclonal
Isotype
Rat (Lewis) IgG2a kappa
Clone Number
18d3
Host
Rat
Species Reactivity
Mouse
Form/Format
Purified (UNLB) Antibody
Specificity
Mouse CD94 receptor subunit, Mr 70 kDa
The monoclonal antibody 18D3 reacts with the mouse CD94 receptor subunit, a 70kDa type II transmembrane glycoprotein. CD94 is expressed on the surface of NK cells, NK T cells and some memory phenotype CD8+ cells.2 When CD94 is paired with NKG2A, C, or E subunit, it binds the nonclassical MHC class Ib molecule Qa-1b and results in a CD94high population by flow cytometry.1, 2 Approximately half of NK cells do not express NKG2A, C or E; these cells express CD94 on the surface in a form that does not bind Qa-1b and is believed to be nonfunctional, producing a CD94low population by flow cytometry.1, 2 CD94 is thought to play a role in the adhesion and activation of the NK cell lineage.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
Molecular Weight
20,513 Da
Chromosome Location
Chromosome: 12; NC_000012.11 (10457050..10469850). Location: 12p13
OMIM
602894
3D Structure
ModBase 3D Structure for Q13241
Immunogen
CHO cells transfected with the B6 allele of CD94
Characterization
To ensure acceptable performance, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent.
Applications Tested/Suitable for anti-KLRD1 antibody
Flow Cytometry
Application Notes for anti-KLRD1 antibody
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells

Product References and Citations for anti-KLRD1 antibody

1. Vance, R.E., A.M. Jamieson, and D.H. Raulet. 1999. J. Exp. Med. 190:1801.
2. Liu, S., and D.H. Raulet. Personal communication.

Other Notes

Small volumes of anti-KLRD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KLRD1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
CD94
NCBI Official Synonym Full Names
killer cell lectin-like receptor subfamily D, member 1
NCBI Official Symbol
KLRD1
NCBI Official Synonym Symbols
CD94
UniProt Protein Name
Natural killer cells antigen CD94
UniProt Synonym Protein Names
KP43; Killer cell lectin-like receptor subfamily D member 1; NK cell receptor
UniProt Gene Name
KLRD1
UniProt Synonym Gene Names
CD94
UniProt Entry Name
KLRD1_HUMAN
NCBI Protein Information
natural killer cells antigen CD94; KP43; CD94 antigen; NK cell receptor; OTTHUMP00000238754; OTTHUMP00000238755; OTTHUMP00000238756; OTTHUMP00000238758; OTTHUMP00000239093

NCBI Summary for KLRD1

Natural killer (NK) cells are a distinct lineage of lymphocytes that mediate cytotoxic activity and secrete cytokines upon immune stimulation. Several genes of the C-type lectin superfamily, including members of the NKG2 family, are expressed by NK cells and may be involved in the regulation of NK cell function. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]
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UniProt Comments for KLRD1

Function: Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells.

Subunit structure: Can form disulfide-bonded heterodimer with NKG2 family members. Ref.8 Ref.9 Ref.10

Subcellular location: Membrane; Single-pass type II membrane protein.

Tissue specificity: Natural killer cells.

Sequence similarities: Contains 1 C-type lectin domain.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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