Erythroid Cell (Ly 76) Antibody – Rat Monoclonal

anti-mouse erythroid cell (Ly-76) mAb is selectively reactive with both fetal and adult erythroid cells. This monoclonal antibody (clone: TER-119) is specific for cells at stages from early proerythroblast to mature erythrocytes. TER-119 is reported to react with 20-25% of bone marrow cells and 2-3% of spleen cells but not with thymocytes or lymph node cells. In fetal haematopoietic tissues, 30-40% of day 10 yolk sac cells, 80-90% of day 14 fetal liver cells and 40-50% of newborn liver cells were reactive with MBS520284. TER-119+ cells in adult bone marrow expressed significant levels of CD45 but not myeloid (Mac-1, Gr-1) or B cell (B220) markers. This mAb immunoprecipitated protein bands with molecular weights of 110 kDa, 60 kDa, 52 kDa and 32 kDa from erythrocyte membrane whereas only a 52 kDa band was detected by TER-119 in Western Blot analysis. It has been determined that the TER-119 antigen is a molecule associated with cell-surface glycophorin A but not with glycophorin A itself. Also the antigen is only expressed on normal erythroid cells but not on erythroleukaemia cells. This product is suitable for use in flow cytometry and immunohistochemistry (Frozen and Paraffin sections).

Small volumes of anti-Erythroid Cell (Ly 76) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS520284 is an antibody targeting Erythroid Cell (Ly 76). Also known as Anti-Mouse Erythroid Cell (Ly 76), FITC (Clone TER119) (rat IgG2b). Reactive in Mouse. Host: Rat.