Mouse Anti-PF4V1 Monoclonal Antibody – Cat# MBS520552

Platelet Factor 4 (PF4V1) Antibody – Mouse Monoclonal

Name: PF4V1 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS520552
Price: 590 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS520552

Description: Platelet factor IV is a 70 aa protein released from the alpha granules of activated platelets. PF4 is synthesized by bone marrow megakaryocytes and stored in alpha granules as a non-covalent bound tetramer. Platelet factor IV binds with high… More details

$590 / 0.2 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Platelet Factor 4 (PF4V1), Monoclonal Antibody

Full Product Name

Anti-Human Platelet Factor 4 (requires heparin for binding),Purified (Clone: KKO) (Mouse IgG2b)

Product Gene Name

anti-PF4V1 antibody

Product Synonym Names

Platelet Factor 4 (requires heparin for binding),Purified (Clone: KKO) (Mouse IgG2b); Anti-Human Platelet Factor IV/Heparin

Clonality

Monoclonal

Isotype

Mouse IgG_2bκ

Clone Number

KKO

Host

Mouse

Species Reactivity

Human

Form/Format

Purified; IgG buffered in PBS and 0.02% NaN3. (Purified from ascitic fluid via Protein G Chromatography). For maximum recovery of contents, spin down tube before use.

Concentration

1.0 mg/ml (lot specific)

Specificity

This antibody is specific for Human Platelet Factor IV /heparin complexes.

Preparation and Storage

For all formats, store at 4°C. For long term storage (Purified, Biotin, HRPO), aliquot and freeze unused portion at -20°C in volumes appropriate for single usage. Avoid freeze/thaw cycles.

Chromosome Location

Chromosome: 4; NC_000004.11 (74719013..74720198). Location: 4q12-q21

OMIM

173461

3D Structure

ModBase 3D Structure for P10720

Immunogen

Human Factor IV purified from the supernatant of thrombin activated platelets.

Presentation

Purified IgG buffered in PBS and 0.02% NaN3. (Purified from ascetic fluid via Protein G Chromatography). For maximum recovery of contents, spin down tube before use.

Applications Tested/Suitable for anti-PF4V1 antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-PF4V1 antibody

This antibody is suitable for use in ELISA and Western Blot. In ELISA use at a concentration of 0.08ug/ml following coating the plate with PF4/heparin complexes. This can be done by incubating PF4 with an excess of heparin (100-200 U/ml), while coating the plate.

Other Notes

Small volumes of anti-PF4V1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PF4V1 purchase

MBS520552 is an antibody targeting PF4V1. Also known as Anti-Human Platelet Factor 4 (requires heparin for binding),Purified (Clone: KKO) (Mouse IgG2b). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PF4V1. It may not necessarily be applicable to this product.

NCBI GI #

292390

NCBI GeneID

5197

NCBI Accession #

AAA60067.1

UniProt Primary Accession #

P10720

UniProt Secondary Accession #

A1L4S0

UniProt Related Accession #

P10720

NCBI Official Full Name

platelet factor 4

NCBI Official Synonym Full Names

platelet factor 4 variant 1

NCBI Official Symbol

PF4V1

NCBI Official Synonym Symbols

PF4A; CXCL4L1; CXCL4V1; PF4-ALT; SCYB4V1

UniProt Protein Name

Platelet factor 4 variant

UniProt Synonym Protein Names

C-X-C motif chemokine 4 variant; CXCL4L1; PF4alt; PF4var1

Protein Family

Platelet factor

UniProt Gene Name

PF4V1

UniProt Synonym Gene Names

CXCL4V1; SCYB4V1

UniProt Entry Name

PF4V_HUMAN

NCBI Protein Information

platelet factor 4 variant; PF4alt; PF4var1; C-X-C motif chemokine 4; platelet factor 4, variant 1 (PF4-like)

UniProt Comments for PF4V1

Function: Inhibitor of angiogenesis. Inhibitor of endothelial cell chemotaxis (in vitro). Ref.5

Subunit structure: Homotetramer. Ref.6

Subcellular location: Secreted.

Post-translational modification: The N-terminal processed forms of platelet factor 4 variant seems to be produced by proteolytic cleavage. The most abundant form is Platelet factor 4 variant(5-74).

Miscellaneous: Binding to heparin is much weaker than in the close homolog PF4/CXCL4.

Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.

Mass spectrometry: Molecular mass is 8250.9 Da from positions 31 - 104. Determined by ESI. Platelet factor 4 variant. Ref.5Molecular mass is 7877.7 Da from positions 34 - 104. Determined by ESI. Platelet factor 4 variant(4-74). Ref.5Molecular mass is 7805.8 Da from positions 35 - 104. Determined by ESI. Platelet factor 4 variant(5-74). Ref.5Molecular mass is 7678.6 Da from positions 36 - 104. Determined by ESI. Platelet factor 4 variant(6-74). Ref.5

Research Articles on PF4V1

1. Structural insight into the novel functional properties of human CXCL4L1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PF4V1 antibody

Products by Pathway	Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Common Pathway antibodies	Common Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies	Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram

Diseases associated with anti-PF4V1 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>6 publications with PF4V1 and Inflammation
Carcinoma Antibodies	>4 publications with PF4V1 and Carcinoma
Adenocarcinoma Antibodies	>3 publications with PF4V1 and Adenocarcinoma
Neoplasms, Experimental Antibodies	>3 publications with PF4V1 and Neoplasms, Experimental
Vascular Diseases Antibodies	>2 publications with PF4V1 and Vascular Diseases
Necrosis Antibodies	>2 publications with PF4V1 and Necrosis
Coronary Thrombosis Antibodies	>1 publications with PF4V1 and Coronary Thrombosis
Thrombophilia Antibodies	>1 publications with PF4V1 and Thrombophilia
Liver Neoplasms Antibodies	>1 publications with PF4V1 and Liver Neoplasms
Heart Diseases Antibodies	>1 publications with PF4V1 and Heart Diseases