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CD200 Antibody – Rat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS520896

CD200, also known as OX-2, is a type-1 membrane glycoprotein that belongs to the immunoglobin superfamily (IgSF). It's expressed on several tissues including thymocytes, neurons, endothelium, follicular dendritic cells in all lymphoid organs, a subset of… More details

$580 / 0.25 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD200, Antibody
Full Product Name
Anti-Human CD200, Purified, (Clone: 1B9) (Rat IgG2a)
Product Gene Name
Isotype
IgG2a
Clone Number
1B9
Host
Rat
Species Reactivity
Human
Form/Format
Purified
Concentration
1.0 mg/ml (lot specific)
Specificity
This antibody is specific for human CD200.
Preparation and Storage
Store at 4°C.
DO NOT FREEZE APC, PE, AF488, and AF647 conjugates.
For long term storage (Purified, LE, Biotin and FITC), aliquot and freeze unused portion -20°C in volumes appropriate for single usage.
Avoid freeze/thaw cycles.
Immunogen
Human CD200-Fc fusion protein.
Conjugate
Unconjugated
Presentation
Purified: Purified IgG buffered in PBS AND 0.02% NaN3. (Purified via Protein G Chromatography). For maximum recovery of contents, spin down tube before use.
LE: Purified Ig buffered in PBS, no preservative, 0.2um sterile filtered. (Purified from cell culture supernatant via Protein G Chromataopgraphy)
Biotin, FITC, PE, APC, AF488 and AF647: Biotin/FITC/PE/APC/AF488/AF647 conjugated IgG buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabiizing protein to bring total protein concentration to 4-5 mg/ml.
Applications Tested/Suitable for anti-CD200 antibody
ELISA (EIA), Flow Cytometry (FC)
Application Notes for anti-CD200 antibody
Tissue Distribution by Flow Cytometry Analysis:
Cell Concentration: 1x106 cells per tests
Antibody Concentration Used: 0.1 ug/106 cells

Cell Source: Human PBLs

Percentage of cells stained above control: 16.4 %

*For optimal results in various applications, it is recommended that each investigator determine dilutions appropriate for individual use.

Flow Cytometry of anti-CD200 antibody

Human peripheral blood lymphocytes were stained with anti-CD200 (clone: 1B9) (blue histogram) or Rat IgG2a isotype control (red histogram).
Rat Anti-CD200 Antibody – Cat# MBS520896

Other Notes

Small volumes of anti-CD200 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD200 purchase

MBS520896 is an antibody targeting CD200. Also known as Anti-Human CD200, Purified, (Clone: 1B9) (Rat IgG2a). Reactive in Human. Host: Rat.

Additional Details

NCBI/Uniprot data below describe general gene information for CD200. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
NCBI Official Full Name
CD200 antigen, isoform CRA_a
NCBI Official Synonym Full Names
CD200 molecule
NCBI Official Symbol
CD200
NCBI Official Synonym Symbols
MRC; MOX1; MOX2; OX-2
NCBI Protein Information
OX-2 membrane glycoprotein

NCBI Summary for CD200

This gene encodes a type I membrane glycoprotein containing two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. This gene is expressed by various cell types, including B cells, a subset of T cells, thymocytes, endothelial cells, and neurons. The encoded protein plays an important role in immunosuppression and regulation of anti-tumor activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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