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RBC Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS524365

This product is rabbit IgM directed against mouse red blood cells. This IgM fraction was isolated fiom a high titer antiserum by salt fractionation and DEAE ion exchange chromatography. It was titered against its immunogens, sterile filtered, and analyzed for… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
RBC, Polyclonal Antibody
Full Product Name
Anti-Mouse RBC, IgM Fraction, (Polyclonal) (rabbit IgM)
Product Gene Name
Clonality
Polyclonal
Isotype
IgM
Host
Rabbit
Species Reactivity
Mouse
Form/Format
5 mg of IgM fraction in 0.5M NaCl, 0.01M phosphate.
Concentration
0.75 mg IgM/ml (lot specific)
Specificity
RBCs
Preparation and Storage
Divide into useful aliquots and store at 2-8°C.
Preservatives
0.2 um sterile filtered; contains 0.05% sodium azide.
Hemagglutination
- Test Cell Suspension: 100 ul of washed 1.5% fresh mouse RBCs in saline.
- Product Tested: Diluted antibody (lot#: 0192M-1) in 100 ul of heat inactivated normal rabbit serum diluted 1:100 in saline.
- Incubation: 37°C for 20 mins, in U-bottom microtiter plates, 20°C for 2 to 4 hrs. for cell settling pattern
- Titer: The highest dilution of the product that yields agglutination.
- Results: Titer of 1:400
Ouchterlony
- Sample Size: 25 ul
- Distance between wells: 4 mm
- Test time: 24 hr.
- Antiserum tested: Goat anti-rabbit IgM and Goat anti-rabbit IgG
- Results: Positive for IgM and Negative for IgG

Product Categories/Family for anti-RBC antibody

Applications Tested/Suitable for anti-RBC antibody
Cytotoxicity Assay (CT)
Application Notes for anti-RBC antibody
Optimum conditions for use of this antibody in analytical procedures should be established in each laboratory.

Other Notes

Small volumes of anti-RBC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RBC purchase

MBS524365 is an antibody targeting RBC. Also known as Anti-Mouse RBC, IgM Fraction, (Polyclonal) (rabbit IgM). Reactive in Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RBC. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Official Full Name
Ribulose bisphosphate carboxylase
NCBI Official Symbol
TK_RS11535
UniProt Protein Name
Ribulose bisphosphate carboxylase
UniProt Gene Name
rbcL
UniProt Synonym Gene Names
RuBisCO
UniProt Entry Name
RBL_THEKO
NCBI Protein Information
ribulose bisophosphate carboxylase

UniProt Comments for RBC

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA) (PubMed:9988755, PubMed:10512715). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase (PubMed:17303759).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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