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His Tag Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS525001

MBS525001 is an antibody targeting His Tag. Also known as Anti-His Tag (HIS.H8) Monoclonal Antibody. Host: Mouse. More details

$245 / 0.05 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
His Tag, Monoclonal Antibody
Full Product Name
Anti-His Tag (HIS.H8) Monoclonal Antibody
Product Synonym Names
Anti-His Tag
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
HIS.H8
Host
Mouse
Sensitivity
WB (with ECL): 1-2ng of His-tagged fusion proteins
Form/Format
1 mg/ml in 10mM PBS, pH 7.2, with 0.05% NaN3
Purity/Purification
Protein A affinity chromatography from mouse ascites fluid
Specificity
Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
Preparation and Storage
Store at -20 degree C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4 degree C for several days to weeks.
Immunogen
HHHHHH (6x His) synthetic peptide conjugated to KLH

Product Categories/Family for anti-His Tag antibody

Epitope Tag mAbs
Applications Tested/Suitable for anti-His Tag antibody
Dot Blot (DB), ELISA (EIA), Immunoprecipitation (IP), Immunostaining (IS), Western Blot (WB)
Application Notes for anti-His Tag antibody
WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature).
IS: 1:500-2000 dilution
IP: 1:500-200 dilution
For best results with other assays (e.g.: Dot, ELISA, etc), please determine optimal working dilutions by titration test

Testing Data of anti-His Tag antibody

Fig. 01 LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)
Mouse Anti-His Tag Monoclonal Antibody – Cat# MBS525001

Testing Data #2 of anti-His Tag antibody

Fig. 02 Comparison between anti-His tag (HIS.H8) mAb with 2 different vendor Abs, probed against a standard ladder (Qiagen Cat. No. 34705) containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5ug/mL)
Mouse Anti-His Tag Monoclonal Antibody, Testing Data #2 – Cat# MBS525001

Testing Data #3 of anti-His Tag antibody

Fig. 03 Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2)
Mouse Anti-His Tag Monoclonal Antibody, Testing Data #3 – Cat# MBS525001

Other Notes

Small volumes of anti-His Tag antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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