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Drebrin 1 (DBN1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380829

MBS4380829 is an antibody targeting DBN1. Also known as Drebrin 1 (DBN1). Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Drebrin 1 (DBN1), Monoclonal Antibody
Full Product Name
Drebrin 1 (DBN1)
Product Gene Name
Product Synonym Names
Developmentally regulated brain protein; Drebrin 1; Drebrin; Drebrin E; Drebrin E2; Drebrin1; DrebrinE
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
DBN1/2879
Host
Mouse
Species Reactivity
Human
Form/Format
200 ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide.
Specificity
Drebrins (developmentally regulated brain proteins) are cytoplasmic proteins that bind F-actin in the brain and are involved in cell migration, extension of neuronal processes and plasticity of dendrites. There are three isoforms: two embryonic types (E1 and E2); and an adult type (A), generated by alternative RNA splicing from a single Drebrin gene. Drebrins are expressed mainly in brain neurons but are also found in skeletal muscle, heart, placenta, pancreas and kidney. Drebrin has been designated as a marker of the dendritic spine. Decreases in Drebrin levels in the brain have been been associated with Alzheimer s disease and Down syndrome.
Preparation and Storage
Antibody with azide: store at -2-8°C. Non-hazardous. No MSDS required.
Molecular Weight
120kDa
OMIM
126660
3D Structure
ModBase 3D Structure for Q16643
Positive Control
kidney and pancreas., PC3 cells. Brain neurons. Also found in the heart, Placenta, Skeletal muscle
Immunogen
Recombinant fragment (aa 150-281) of human DBN1 protein (exact sequence is proprietary)
Cellular Localization
Cell cortex, Cell junction, Cell projection, Cytoplasm, Dendrite, Growth cone
Applications Tested/Suitable for anti-DBN1 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-DBN1 antibody
Western Blot:
(2-4ug/ml)

Immunohistochemistry (Formalin-fixed):
(1-2ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)

Optimal dilution for a specific application should be determined

Immunohistochemistry (IHC) of anti-DBN1 antibody

Formalin-fixed, paraffin-embedded human Brain stained with Drebrin-1 Mouse Monoclonal Antibody (DBN1/2879).
Mouse Anti-DBN1 Monoclonal Antibody – Cat# MBS4380829

Immunohistochemistry (IHC) of anti-DBN1 antibody

Formalin-fixed, paraffin-embedded human Cervix stained with Drebrin-1 Mouse Monoclonal Antibody (DBN1/2879).
Mouse Anti-DBN1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4380829

SDS-PAGE of anti-DBN1 antibody

SDS-PAGE Analysis Purified Drebrin-1 Mouse Monoclonal Antibody (DBN1/2879). Confirmation of Integrity and Purity of Antibody
Mouse Anti-DBN1 Monoclonal Antibody, SDS-PAGE – Cat# MBS4380829

Testing Data of anti-DBN1 antibody

Analysis of Protein Array containing more than 19,000 full-length human proteins using Drebrin-1 Mouse Monoclonal Antibody (DBN1/2879). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
Mouse Anti-DBN1 Monoclonal Antibody, Testing Data – Cat# MBS4380829

Western Blot (WB) of anti-DBN1 antibody

Western Blot Analysis of PC3 cell lysate using Drebrin-1 Mouse Monoclonal Antibody (DBN1/2879)
Mouse Anti-DBN1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS4380829

Product References and Citations for anti-DBN1 antibody

Shirao, T. 1995. The roles of microfilament-associated proteins, drebrins, in brain morphogenesis: a review. J. Biochem. 117: 231-236.

Other Notes

Small volumes of anti-DBN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DBN1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
drebrin isoform a
NCBI Official Synonym Full Names
drebrin 1
NCBI Official Symbol
DBN1
NCBI Official Synonym Symbols
D0S117E
UniProt Protein Name
Drebrin
UniProt Synonym Protein Names
Developmentally-regulated brain protein
Protein Family
UniProt Gene Name
DBN1
UniProt Synonym Gene Names
D0S117E
UniProt Entry Name
DREB_HUMAN
NCBI Protein Information
drebrin

NCBI Summary for DBN1

The protein encoded by this gene is a cytoplasmic actin-binding protein thought to play a role in the process of neuronal growth. It is a member of the drebrin family of proteins that are developmentally regulated in the brain. A decrease in the amount of this protein in the brain has been implicated as a possible contributing factor in the pathogenesis of memory disturbance in Alzheimer's disease. At least two alternative splice variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for DBN1

DBN1: a cytoplasmic actin-binding protein thought to play a role in the process of neuronal growth. It is a member of the drebrin family of proteins that are developmentally regulated in the brain. A decrease in the amount of this protein in the brain has been implicated as a possible contributing factor in the pathogenesis of memory disturbance in Alzheimer's disease. Expressed in brain neurons, heart, placenta, skeletal muscle, kidney and pancreas. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Actin-binding; Cytoskeletal

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: cytoplasm; dendrite; plasma membrane; gap junction; actomyosin; cell cortex; actin cytoskeleton

Molecular Function: protein binding; actin binding; profilin binding

Biological Process: maintenance of cellular protein localization; regulation of dendrite development; actin filament organization; regulation of neuronal synaptic plasticity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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