Cortisol 3 Antibody – Rabbit

Rabbit polyclonal antibodies against cortisol 3 were developed using BSA-conjugated cortisol 3 (cortisol-3 -CMO-BSA) as immunogen.

Cortisol is steroid hormone from group of glucocorticoid hormones; it’s produced by adrenal gland in response to stress and low concentration of glucose in blood. Its primary functions include activation of gluconeogenesis to increase of blood glucose level, participation in metabolism of carbohydrates, proteins and lipids, and suppression of immune system.

Activity of these polyclonal anti-cortisol antibodies is tested by I125RIA; its’ measured ED50 value is 68ng/mL (lot-specific) and titer is up to 100,000. Reported affinity constant is 9.0*109 L/M.

Two grades of antibodies are available with some lots recommended for coating applications.

Comparative reactivity of antibodies against related steroid hormones are as following: 100% for cortisol, 36.0% for prednisolone, 5.7% for 11-desoxycortisol, 3.3% for corticosterone and less than 0.7% for cortisone.

These polyclonal antibodies are not purified and are supplied as immune serum without added preservatives; volume of aliquot is up to 0.4mL (lot-specific) with estimated potency of 15,000 tests/mL.

Antibodies must be stored refrigerated at 4°C for short-term storage, and aliquoted and stored frozen below -20°C for long-term storage; repetitive freeze-thaw cycles must be avoided.

QTL affecting morphometric traits and stress response in the gilthead seabream (Sparus aurata); Kay Boultona, Cecile Massaulta, Ross D. Houstona, Dirk Jan de Koninga, Chris S. Haleya, Henk Bovenhuisb, Costas Batargiasd, Adelino V.M. Canariof, Giorgios Kotoulasd, Costas S. Tsigenopoulos (Aquaculture: Volume: 319 Issue: 1-2 Page: 58-66 DOI: 10.1016/j.aquaculture.2011.06.044)
Health anxiety moderates the daytime cortisol slope; Eamonn Ferguson (Journal of Psychosomatic Research: Volume: 64 Issue: 5 Page: 487-494 DOI: 10.1016/j.jpsychores.2008.01.011)

Small volumes of anti-Cortisol 3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.