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cdc25A Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS5309539

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase ofthe cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specificallydegraded in response to… More details

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Product Overview

Online Datasheet in PDF
Product Name
cdc25A, Polyclonal Antibody
Full Product Name
cdc25A antibody
Product Gene Name
Product Synonym Names
Rabbit polyclonal cdc25A antibody; Polyclonal cdc25A antibody; Anti-cdc25A antibody; Cell division cycle 25A antibody; cdcA 25 antibody; Phospho Ser76 antibody; Cell division cycle 25 homolog A antibody; cdcA-25 antibody; cdcA 25; cdcA-25; cdc25A
Clonality
Polyclonal
Host
Rabbit
Form/Format
Liquid form in PBS buffer (no MG2+ or Ca2+), pH 7.4, 150 mm NaCl,0.02% sodium azide and 50% glycerol.
Purity/Purification
Purified by immunoaffinity chromatography.
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C for short term storage.
Aliquot and store at-20 degree C for long term storage.
Avoid repeated freeze/thaw cycles.
Molecular Weight
54,551 Da
OMIM
116947
Immunogen
cdc25A antibody was raised in Rabbit using the peptide sequence around phosphorylation site of serine 76 (M-G-S(p)-S-E) derived from Human cdc25A as the immunogen.
Cross-Reactivity
Human, Mouse, Rat

Product Categories/Family for anti-cdc25A antibody

Applications Tested/Suitable for anti-cdc25A antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-cdc25A antibody
IHC: 1:50-1:100
WB: 1:500-1:1000

Immunohistochemistry (IHC) of anti-cdc25A antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25A (Phospho-Ser76) antibody (left) or the same antibody preincubated with with blocking peptide (right)
Rabbit Anti-cdc25A Polyclonal Antibody – Cat# MBS5309539

Western Blot (WB) of anti-cdc25A antibody

Western Blot analysis of extracts from Hela cells untreated or treated with TNF-a and CA using cdc25A (Phospho-Ser76) antibody
Rabbit Anti-cdc25A Polyclonal Antibody, Western Blot (WB) – Cat# MBS5309539

Other Notes

Small volumes of anti-cdc25A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for cdc25A purchase

MBS5309539 is an antibody targeting cdc25A. Also known as cdc25A antibody. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for cdc25A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
CDC25A, partial
NCBI Official Synonym Full Names
cell division cycle 25A
NCBI Official Symbol
CDC25A
NCBI Official Synonym Symbols
CDC25A2
UniProt Protein Name
M-phase inducer phosphatase 1
UniProt Synonym Protein Names
Dual specificity phosphatase Cdc25A
UniProt Gene Name
CDC25A
NCBI Protein Information
M-phase inducer phosphatase 1

NCBI Summary for cdc25A

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for cdc25A

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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