Western Blot of GFP antibody. Lane 1: Molecular Weight Markers. Lane 2: GFP. Load: 10ng per lane. Ms Anti-GFP antibody at 1:1000 for overnight at 4 deg C. Mouse HRP secondary antibody at 1:40,000 for 30 min at RT. Block: 5% Blocking buffer overnight at 4 deg C. Size: 27 kDa, 27 kDa for epitope tag GFP..

Western Blot of GFP antibody. Lane 1: 64pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 2: 32pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 3: 16pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 4: 8pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 5: 4pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 6: 2pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 7: 1g of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 8: 0pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. anti-GFP monoclonal antibody at 1:400 for overnight at 4 deg C. HRP-conjugated anti-Mouse IgG was performed at a dilution of 1:20,000 for 1h at 4 deg C. Block: TTBS supplemented with 1% BSA for 1 h at 4 deg C. Size: 27 kDa for GFP..