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Mad2L1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS5311968

MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been… More details

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Product Overview

Online Datasheet in PDF
Product Name
Mad2L1, Polyclonal Antibody
Full Product Name
Mad2L1 Antibody
Product Gene Name
anti-Mad2L1 antibody
Product Synonym Names
Affinity Purified Rabbit Polyclonal Mad2L1 Antibody; Polyclonal Mad2L1 antibody; Anti-Mad2L1 antibody; MadL1 2 antibody; HSMAD2 antibody; MAD2L1 antibody; MAD2 (mitotic arrest deficient yeast homolog)-like 1 antibody; MadL1 2; mitotic spindle assembly checkpoint protein MAD2A antibody; MAD2 antibody; mitotic arrest deficient yeast homolog-like 1 antibody; MAD2-like protein 1 antibody; MadL1-2 antibody; MadL1-2; Mad2L1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Supplied as a concentrated soloution containing 1X PBS, 40% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Purity/Purification
Antigen-Affinity Chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Molecular Weight
24 kDa
OMIM
601467
3D Structure
ModBase 3D Structure for Q13257
Immunogen
Mad2L1 antibody was raised in rabbit using a synthetic peptide corresponding to a region within amino acids 142 and 205 of Mad2L1
Cross-Reactivity
Human

Product Categories/Family for anti-Mad2L1 antibody

Proteases; Inhibitors; & Enzymes
Applications Tested/Suitable for anti-Mad2L1 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-Mad2L1 antibody
IF: 1:100-1:200
ICC: 1:100-1:200
IHC-P: 1:100-1:500
WB: 1:500-1:3000

Immunofluorescence (IF) of anti-Mad2L1 antibody

Immunofluorescence analysis of human osteosarcoma cell line U2OS, using MAD2L1 antibody at 1:1000 dilution.
Rabbit Anti-Mad2L1 Polyclonal Antibody – Cat# MBS5311968

Immunohistochemistry (IHC) of anti-Mad2L1 antibody

Immunohistochemical staining of paraffin-embedded DLD-1 xenograft using Mad2L1 antibody at a dilution of 1:500
Rabbit Anti-Mad2L1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS5311968

Western Blot (WB) of anti-Mad2L1 antibody

Western blot analysis of 30 ug whole cell lysate (A:Hep G2; B:MOLT4; C:Raji) using a 12% SDS PAGE gel and Mad2L1 antibody at a dilution of 1:1000
Rabbit Anti-Mad2L1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS5311968

Other Notes

Small volumes of anti-Mad2L1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Mad2L1 purchase

MBS5311968 is an antibody targeting Mad2L1. Also known as Mad2L1 Antibody. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Mad2L1. It may not necessarily be applicable to this product.

NCBI GI #
4505067
NCBI GeneID
4085
UniProt Primary Accession #
Q13257
UniProt Related Accession #
Q13257
GenBank Nucleotide #
NP_002349.1
NCBI Official Full Name
mitotic spindle assembly checkpoint protein MAD2A
NCBI Official Synonym Full Names
mitotic arrest deficient 2 like 1
NCBI Official Symbol
MAD2L1
NCBI Official Synonym Symbols
MAD2; HSMAD2
UniProt Protein Name
Mitotic spindle assembly checkpoint protein MAD2A
UniProt Synonym Protein Names
Mitotic arrest deficient 2-like protein 1
Protein Family
Mitotic spindle assembly checkpoint protein
UniProt Gene Name
MAD2L1
UniProt Synonym Gene Names
MAD2; HsMAD2
UniProt Entry Name
MD2L1_HUMAN
NCBI Protein Information
mitotic spindle assembly checkpoint protein MAD2A

UniProt Comments for Mad2L1

MAD2L1: required for the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. Inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. Interacts with Cdc20, MAD2L1BP and with ADAM17/TACE

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 4q27

Cellular Component: kinetochore; spindle pole; perinuclear region of cytoplasm; nuclear pore; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; protein homodimerization activity

Biological Process: negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitotic sister chromatid segregation; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; negative regulation of mitotic cell cycle; mitotic cell cycle; negative regulation of protein catabolic process; negative regulation of apoptosis
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Research Articles on Mad2L1

1. Low expression of BRCA1 and its transcriptional target MAD2 expression are strongly correlated with improved survival in high-grade serous carcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Mad2L1 antibody

 Reference Product  PubMed Publications
 CDC20 antibody  >82 publications with Mad2L1 and CDC20
 MAD1L1 antibody  >44 publications with Mad2L1 and MAD1L1
 MAD2L2 antibody  >23 publications with Mad2L1 and MAD2L2
 MAD2L1BP antibody  >12 publications with Mad2L1 and MAD2L1BP
 NDC80 antibody  >7 publications with Mad2L1 and NDC80
 UBD antibody  >3 publications with Mad2L1 and UBD
 E2F1 antibody  >3 publications with Mad2L1 and E2F1
 CDC27 antibody  >1 publications with Mad2L1 and CDC27
 CDC16 antibody  >1 publications with Mad2L1 and CDC16
 E2F4 antibody  >1 publications with Mad2L1 and E2F4

Pathways associated with anti-Mad2L1 antibody

 Products by Pathway  Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 Amplification Of Signal From Unattached Kinetochores Via A MAD2 Inhibitory Signal Pathway antibodies  Amplification Of Signal From Unattached Kinetochores Via A MAD2 Inhibitory Signal Pathway Diagram
 Amplification Of Signal From The Kinetochores Pathway antibodies  Amplification Of Signal From The Kinetochores Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram

Diseases associated with anti-Mad2L1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >111 publications with Mad2L1 and Neoplasms
 Carcinoma Antibodies  >52 publications with Mad2L1 and Carcinoma
 Adenocarcinoma Antibodies  >27 publications with Mad2L1 and Adenocarcinoma
 Breast Neoplasms Antibodies  >17 publications with Mad2L1 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >13 publications with Mad2L1 and Cell Transformation, Neoplastic
 Liver Diseases Antibodies  >10 publications with Mad2L1 and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >10 publications with Mad2L1 and Carcinoma, Hepatocellular
 Neoplasm Metastasis Antibodies  >8 publications with Mad2L1 and Neoplasm Metastasis
 Ovarian Neoplasms Antibodies  >8 publications with Mad2L1 and Ovarian Neoplasms
 Liver Neoplasms Antibodies  >8 publications with Mad2L1 and Liver Neoplasms

Organs/Tissues associated with anti-Mad2L1 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >11 publications with Mad2L1 and Lung
 Stomach Antibodies  >10 publications with Mad2L1 and Stomach
 Liver Antibodies  >10 publications with Mad2L1 and Liver
 Prostate Antibodies  >7 publications with Mad2L1 and Prostate
 Blood Antibodies  >7 publications with Mad2L1 and Blood
 Bone Antibodies  >6 publications with Mad2L1 and Bone
 Lymph Antibodies  >5 publications with Mad2L1 and Lymph
 Skin Antibodies  >4 publications with Mad2L1 and Skin
 Brain Antibodies  >4 publications with Mad2L1 and Brain
 Kidney Antibodies  >3 publications with Mad2L1 and Kidney
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