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SAE1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS5312429

Posttranslational modification of proteins by the addition of the small protein SUMO, or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
SAE1, Polyclonal Antibody
Full Product Name
SAE1 Antibody
Product Gene Name
anti-SAE1 antibody
Product Synonym Names
Affinity Purified Rabbit Polyclonal SAE1 Antibody; Polyclonal SAE1 antibody; Anti-SAE1 antibody; AOS1 antibody; SUA1 antibody; SUMO-activating enzyme subunit 1 antibody; UBLE1A antibody; SAE1; FLJ3091 antibody; activator of SUMO1 antibody; SAE 1; ubiquitin-like protein SUMO-1 activating enzyme antibody; SAE-1; SAE-1 antibody; HSPC140 antibody; SAE1 antibody; sentrin/SUMO-activating protein AOS1 antibody; SAE 1 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Purity/Purification
Antigen-Affinity Chromatography
Concentration
0.72 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Molecular Weight
38 kDa
OMIM
613294
3D Structure
ModBase 3D Structure for Q9UBE0
Cross-Reactivity
Human, Mouse

Product Categories/Family for anti-SAE1 antibody

Protein Modification & Stress Response
Applications Tested/Suitable for anti-SAE1 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-SAE1 antibody
IF: 1:100-1:500
ICC: 1:100-1:500
IHC-P: 1:100-1:500
WB: 1:500-1:3000

Western Blot (WB) of anti-SAE1 antibody

Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 10% SDS PAGE gel and SAE1 antibody at a dilution of 1:1000
Rabbit Anti-SAE1 Polyclonal Antibody – Cat# MBS5312429

Immunohistochemistry (IHC) of anti-SAE1 antibody

Immunohistochemical staining of paraffin-embedded A549 Xenograft using SAE1 antibody at a dilution of 1:100
Rabbit Anti-SAE1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS5312429

Western Blot (WB) of anti-SAE1 antibody

Western blot analysis of 30 ug of whole cell lysate (A: A431; B: H1299) using a 10% SDS PAGE gel and SAE1 antibody at a dilution of 1:1000
Rabbit Anti-SAE1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS5312429

Immunofluorescence (IF) of anti-SAE1 antibody

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SAE1 antibody at 1:200 dilution.
Rabbit Anti-SAE1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS5312429

Other Notes

Small volumes of anti-SAE1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SAE1 purchase

MBS5312429 is an antibody targeting SAE1. Also known as SAE1 Antibody. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SAE1. It may not necessarily be applicable to this product.

NCBI GI #
225543288
NCBI GeneID
10055
UniProt Primary Accession #
Q9UBE0
UniProt Related Accession #
Q9UBE0
GenBank Nucleotide #
NP_001139186.1
NCBI Official Full Name
SUMO-activating enzyme subunit 1 isoform c
NCBI Official Synonym Full Names
SUMO1 activating enzyme subunit 1
NCBI Official Symbol
SAE1
NCBI Official Synonym Symbols
AOS1; SUA1; UBLE1A; HSPC140
UniProt Protein Name
SUMO-activating enzyme subunit 1
UniProt Synonym Protein Names
Ubiquitin-like 1-activating enzyme E1A
Protein Family
SUMO-activating enzyme
UniProt Gene Name
SAE1
UniProt Synonym Gene Names
AOS1; SUA1; UBLE1A
UniProt Entry Name
SAE1_HUMAN
NCBI Protein Information
SUMO-activating enzyme subunit 1

NCBI Summary for SAE1

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]
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UniProt Comments for SAE1

UBLE1A: The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. Belongs to the ubiquitin-activating E1 family.

Protein type: EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytosol; nucleus

Molecular Function: protein C-terminus binding; ATP-dependent protein binding; SUMO activating enzyme activity; protein binding; protein heterodimerization activity; enzyme activator activity; ubiquitin activating enzyme activity

Biological Process: positive regulation of catalytic activity; cellular protein metabolic process; protein sumoylation; regulation of mitotic cell cycle; protein ubiquitination; SMT3-dependent protein catabolic process; post-translational protein modification
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Research Articles on SAE1

1. the expression of SENP8, SAE1, PIAS1, PIAS2 and ZMIZ1 is deregulated in the majority of PTC tissues, likely contributing to the PTC phenotype.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SAE1 antibody

 Reference Product  PubMed Publications
 SUMO1 antibody  >28 publications with SAE1 and SUMO1
 UBA2 antibody  >21 publications with SAE1 and UBA2
 SUMO2 antibody  >4 publications with SAE1 and SUMO2
 SUMO3 antibody  >2 publications with SAE1 and SUMO3
 UBE2I antibody  >2 publications with SAE1 and UBE2I
 RBX1 antibody  >1 publications with SAE1 and RBX1
 UBC antibody  >1 publications with SAE1 and UBC

Pathways associated with anti-SAE1 antibody

 Products by Pathway  Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 Processing And Activation Of SUMO Pathway antibodies  Processing And Activation Of SUMO Pathway Diagram
 SUMO Is Conjugated To E1 (UBA2:SAE1) Pathway antibodies  SUMO Is Conjugated To E1 (UBA2:SAE1) Pathway Diagram
 SUMO Is Transferred From E1 To E2 (UBE2I, UBC9) Pathway antibodies  SUMO Is Transferred From E1 To E2 (UBE2I, UBC9) Pathway Diagram
 SUMOylation Pathway antibodies  SUMOylation Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram

Diseases associated with anti-SAE1 antibody

 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >2 publications with SAE1 and Mental Disorders
 Disease Models, Animal Antibodies  >2 publications with SAE1 and Disease Models, Animal
 Muscular Diseases Antibodies  >1 publications with SAE1 and Muscular Diseases
 Death Antibodies  >1 publications with SAE1 and Death
 Brain Diseases Antibodies  >1 publications with SAE1 and Brain Diseases
 Lung Diseases Antibodies  >1 publications with SAE1 and Lung Diseases

Organs/Tissues associated with anti-SAE1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with SAE1 and Brain
 Muscle Antibodies  >2 publications with SAE1 and Muscle
 Blood Antibodies  >2 publications with SAE1 and Blood
 Skin Antibodies  >2 publications with SAE1 and Skin
 Vascular Antibodies  >1 publications with SAE1 and Vascular
 Connective Tissue Antibodies  >1 publications with SAE1 and Connective Tissue
 Kidney Antibodies  >1 publications with SAE1 and Kidney
 Uterus Antibodies  >1 publications with SAE1 and Uterus
 Embryonic Tissue Antibodies  >1 publications with SAE1 and Embryonic Tissue
 Lung Antibodies  >1 publications with SAE1 and Lung
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