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NFkB Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS5313643

NF-kB functions as a fast messenger. Under normal circumstances, it is sequestered in the cytoplasm by an inhibitor protein. Additionally, the p50 subunit is in the inactive elongated p105 state. Once a viral infection is recognized, the inhibitor is… More details

$785 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NFkB p65, Polyclonal Antibody
Full Product Name
NFkB p65 Antibody (Ser529)
Product Gene Name
anti-NFkB antibody
Product Synonym Names
Rabbit Polyclonal NFkB p65 Antibody (Ser529); Polyclonal NFkB p65 antibody; Anti-NFkB p65 antibody; MGC131774 antibody; NFKB3 antibody; Nuclear factor NF-kappa-B p65 subunit antibody; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody; p65 antibody; RELA antibody; TF65 antibody; Transcription factor p65 antibody; v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65)) antibody; v-rel reticuloendotheliosis viral oncogene homolog A (avian) antibody; v-rel reticuloendotheliosis viral oncogene homolog A nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 p65 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse
Form/Format
Supplied in PBS buffer (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
Purity/Purification
Affinity Chromatography
Concentration
1 mg/ml (lot specific)
Specificity
NFkB p65 antibody (Ser529) detects endogenous levels of NFkB p65 protein only when phosphorylated at serine 529. The antibody will detect species specific phosphorylation as follows: Human: Ser529, Mouse: Ser529, Rat: Ser529
Preparation and Storage
Store at -20 degree C in small aliquots. Avoid repeat freeze/thaw cycles.
Immunogen
NFkB p65 antibody was raised in Rabbit using a synthesized phosphopeptide derived from human NFkB p65 around the phosphorylation site of serine 529 (L-L-SP-G-D) as the immunogen.
Cross-Reactivity
Mouse, Rat

Product Categories/Family for anti-NFkB antibody

Cytokines & Growth Factors
Applications Tested/Suitable for anti-NFkB antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-NFkB antibody
WB: 1:500-1:1000
IHC: 1:50-1:100

Western Blot (WB) of anti-NFkB antibody

Western blot analysis of extracts from MDA-MB-231 cells, untreated or treated with TNF-alpha (20ng/ml, 10min) using NF-kappaB p65 antibody and NFkB p65 antibody (Ser529) (Lanes 3 and 4)
Rabbit Anti-NFkB Polyclonal Antibody – Cat# MBS5313643

Immunohistochemistry (IHC) of anti-NFkB antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFkB p65 antibody (Ser529)
Rabbit Anti-NFkB Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS5313643

Other Notes

Small volumes of anti-NFkB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NFkB purchase

MBS5313643 is an antibody targeting NFkB. Also known as NFkB p65 Antibody (Ser529). Reactive in Mouse. Host: Rabbit.
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