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LDHB Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423349

MBS423349 is an antibody targeting LDHB. Also known as Goat anti-LDHB Antibody. Reactive in Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LDHB, Polyclonal Antibody
Full Product Name
Goat anti-LDHB Antibody
Product Gene Name
Product Synonym Names
Goat Anti-LDHB (aa15-25) Antibody; LDHB; lactate dehydrogenase B; LDH-B; LDH-H; LDHBD; TRG-5; L-lactate dehydrogenase B chain; LDH heart subunit; lactate dehydrogenase H chain; renal carcinoma antigen NY-REN-46; LDHB antibody; lactate dehydrogenase B antibody; LDH-B antibody; LDH-H antibody; LDHBD antibody; TRG-5 antibody; L-lactate dehydrogenase B chain antibody; LDH heart subunit antibody; lactate dehydrogenase H chain antibody; renal carcinoma antigen NY-REN-46 antibody
Immunogen Sequence
NARGLTSVIN QKLK
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
LDHB peptide (MBS428457) is used for blocking the activity of LDHB antibody (MBS423349)
Molecular Weight
36,638 Da
OMIM
150100
Immunogen
Peptide with sequence C-NARGLTSVINQKLK, from the internal region (near C terminus) of the protein sequence according to NP_002291.1.
Epitope
Internal region (near C Terminus)
Note
Reported variants represent identical protein: NP_002291.1, NP_001167568.1.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-LDHB antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-LDHB antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 35kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 36.6kDa according to NP_002291.1). Recommended concentration: 0. 03-0.1ug/ml.

Western Blot (WB) of anti-LDHB antibody

(0.1ug/ml) staining of Pig Heart lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-LDHB Polyclonal Antibody – Cat# MBS423349

Western Blot (WB) of anti-LDHB antibody

(0.1ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-LDHB Polyclonal Antibody, Western Blot (WB) – Cat# MBS423349

Other Notes

Small volumes of anti-LDHB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for LDHB. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
L-lactate dehydrogenase B chain isoform LDHB
NCBI Official Synonym Full Names
lactate dehydrogenase B
NCBI Official Symbol
LDHB
NCBI Official Synonym Symbols
LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
UniProt Protein Name
L-lactate dehydrogenase B chain
UniProt Synonym Protein Names
LDH heart subunit; LDH-H; Renal carcinoma antigen NY-REN-46
UniProt Gene Name
LDHB
UniProt Synonym Gene Names
LDH-B; LDH-H
UniProt Entry Name
LDHB_HUMAN
NCBI Protein Information
L-lactate dehydrogenase B chain

NCBI Summary for LDHB

This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13. [provided by RefSeq, Feb 2016]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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