Goat Anti-GFP Polyclonal Antibody – Cat# MBS534285

Immunofluorescent staining of GFP antibody
Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus in Mus musculus (coronal view, 20X magnification). Sf-1:Cre mice were crossed to the Z/EG reporter line. Brains were harvested following cardiac perfusion with 4% PFA/PBS with ON fixation, and subsequently transferred to a 30% sucrose solution. Brains were then frozen in OCT freezing medium and cryostat sectioned at 40 microns. GFP antibody was used at 1:500 dilution in free floating imunnohistochemistry to detect eGFP. Fluorchrome conj ugated anti-Goat IgG secondary antibody was used for detection at 1:500. Sections were counterstained with DAPI.

GFP Antibody – Goat Polyclonal

Name: GFP Antibody; Polyclonal; Goat; Jellyfish GFP-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS534285
Price: 2730 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS534285

MBS534285 is an antibody targeting GFP. Also known as GFP antibody. Reactive in jellyfish GFP. Host: Goat. Affinity purified Goat polyclonal GFP antibody More details

$2730 / 1 mg +3 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GFP, Polyclonal Antibody

Full Product Name

GFP antibody

Product Gene Name

anti-GFP antibody

Product Synonym Names

Polyclonal GFP; Anti-GFP; Green Fluorescent Protein

Clonality

Polyclonal

Host

Goat

Species Reactivity

jellyfish GFP

Form/Format

Sterile filtered liquid in 0.02M K3PO4, pH 7.2, with 0.15M NaCl, and 0.01% NaN3.

Purity/Purification

GFP antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml (lot specific)

Specificity

jellyfish GFP

Preparation and Storage

Store vial at -20 degree C prior to opening. Aliquot and freeze at -20 degree C or below for long term storage. This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freeze/thaw cycles.

Biohazard Information

This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Immunogen

GFP antibody was raised in goat using a GFP fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria as the immunogen.

Product Categories/Family for anti-GFP antibody

Purified Polyclonal Antibodies

Applications Tested/Suitable for anti-GFP antibody

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Application Notes for anti-GFP antibody

ELISA: 1:20,000-1:100,000
IHC: 1:1,000-1:5,000
WB: 1:2,000-1:10,000

Other Notes

Small volumes of anti-GFP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GFP. It may not necessarily be applicable to this product.

NCBI GI #

28883209

NCBI Accession #

AAO43180.1

NCBI Official Full Name

green fluorescent protein

Protein Family

Green fluorescent protein

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.