• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

GFP Antibody

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS534795

MBS534795 is an antibody targeting GFP. Also known as GFP antibody (biotin). Reactive in Assay by immunoelectrophoresis resulted in a single precipitation arc against anti-goat serum, anti-biotin and purified and partially purified green fluorescent protein… More details

$2770 / 1 mg +3 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GFP, Antibody
Full Product Name
GFP antibody (biotin)
Product Gene Name
anti-GFP antibody
Product Synonym Names
Polyclonal GFP; Anti-GFP; Green Fluorescent Protein; GFP conjugate
Species Reactivity
Assay by immunoelectrophoresis resulted in a single precipitation arc against anti-goat serum, anti-biotin and purified and partially purified green fluorescent protein (Aequorea victoria) Serum. no reaction was observed against Human, Mouse, and Rat ser
Form/Format
Lyophilized from 20mM potassium phosphate, 150mM sodium chloride, pH 7.2 with 0.01% sodium azide as preservative. Stabilizer: 10mg/ml BSA-immunoglobulin and Protease free. Reconstitute with 1ml of deionized water.
Concentration
1.0mg/ml, lyophilized (lot specific)
Specificity
Jellyfish GFP
Preparation and Storage
Ships ambient or refrigerated. Upon receipt store at 4°C prior to reconstitution. For long term storage, reconstitute, aliquot, and freeze at -20°C. Avoid repeated freeze-thaw cycles.
Immunogen
Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from jellyfish Aequorea victoria.
Tag
Biotin
Method of Purification
GFP antibody (biotin) was purified by affinity chromatography.
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Product Categories/Family for anti-GFP antibody

Conjugated Polyclonal Antibodies
Application Notes for anti-GFP antibody
ELISA: 1:10,000-1:50,000
IHC: 1:1,000-1:5,000
WB: 1:2,000-1:10,000
Immunomicroscopy as well as other antibody-based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.

Immunofluorescence (IF) of anti-GFP antibody

issue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation: 4%PFA/PBS with o/n fixation, and subsequently transferred to a 30% sucrose solution. Antigen retrieval: frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns. Goat anti-GFP was used at 1:500 dilution in free floating imunnohistochemistry to detect GFP. Fluorchrome conjugated Anti-goat IgG secondary antibody was used for detection at 1:500 at 1:10,000 for 45 min at RT. Localization: Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus. Staining: eGFP as green fluorescent signal and sections were counterstained with DAPI.
Anti-GFP Antibody – Cat# MBS534795

Immunofluorescence (IF) of anti-GFP antibody

Tissue: Drosophila melanogaster late stage embryonic central nervous system. Fixation: 0.5% PFA. Antigen retrieval: not required. Anti-GFP antibody at a 1:1,000 for 1 h at RT. AlexaFluor 488 conjugated anti-Goat antibody at 1:300 for 45 min at RT. Panel A: shows a lateral view (ventral left). Panels B and C: shows ventral views of whole mount embryos at 63x magnification (plus 2x digital zoom). In all panels, anterior is up. Staining: tau-GFP cell bodies (large arrowhead) and axons of motorneurons (arrow) and interneurons (small arrowhead) as green fluorescent signal.
Anti-GFP Antibody, Immunofluorescence (IF) – Cat# MBS534795

Western Blot (WB) of anti-GFP antibody

Western blot of Biotin GFP antibody. Lane 1: GFP. Lane 2: None. Load: 50 ng per lane. GFP antibody Biotin conjugated at 1:1,000 for 60 min at RT. Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 28 kDa, 28 kDa for GFP..
Anti-GFP Antibody, Western Blot (WB) – Cat# MBS534795

Western Blot (WB) of anti-GFP antibody

Western Blot of GFP antibody. Lane 1: HeLa cells. Lane 2: mock transfected HeLa cell lysate. Load: 35 ug per lane. GFP antibody at 1 ug/ml for 1 h at room temperature. IRDye 800 conjugated Donkey-a-Goat IgG [H&L] MX7 (605-732-125) secondary antibody at 1:2,500 for 45 min at RT. Block: 5% Blocking buffer overnight at 4 deg C. Size: 27 kDa, 33 kDa for GFP..
Anti-GFP Antibody, Western Blot (WB) – Cat# MBS534795

Other Notes

Small volumes of anti-GFP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GFP. It may not necessarily be applicable to this product.

NCBI GI #
1169893
NCBI Accession #
P42212.1
UniProt Secondary Accession #
Q17104; Q27903; Q93125
NCBI Official Full Name
Green fluorescent protein
UniProt Protein Name
Green fluorescent protein
Protein Family
Green fluorescent protein
UniProt Gene Name
GFP
UniProt Entry Name
GFP_AEQVI

UniProt Comments for GFP

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000