Rabbit Anti-GLB1 Polyclonal Antibody – Cat# MBS535251

Western Blot analysis using beta Galactosidase antibody
Western blotting using Fluorescein conj ugated beta Galactosidase antibody shows a band at ~117 kDa (lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of beta-Gal present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E. coli lysate. Proteins were resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose and blocking using Blocking Buffer for Fluorescent Western Blotting. The membrane was probed with fluorescein conj ugated beta Galactosidase antibody diluted to 1:10, 000. Reaction occurred for 2 hours at room temperature. Molecular weight estimation was made by comparison to a prestained MW marker in lane M. Fluorescence image was captured using the VersaDoc Imaging System developed by Biorad. Other detection systems will yield similar results.

Beta Galactosidase (GLB1) Antibody – Rabbit Polyclonal

Name: GLB1 Antibody; Polyclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS535251
Price: 1145 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS535251

MBS535251 is an antibody targeting GLB1. Also known as beta Galactosidase antibody. Reactive in To be determined by end-user. Host: Rabbit. Rabbit polyclonal beta Galactosidase antibody More details

$1145 / 2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

beta Galactosidase (GLB1), Polyclonal Antibody

Full Product Name

beta Galactosidase antibody

Product Gene Name

anti-GLB1 antibody

Product Synonym Names

Polyclonal beta Galactosidase; Anti-beta Galactosidase; B-Gal; beta-Galactosidase; BGAL; B-Galactosidase

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

To be determined by end-user

Form/Format

Monospecific antisera, absorbed, delipidated and defibrinated, supplied in 0.02M NaPO4, 0.15M NaCl, pH 7.2, with 0.01% NaN3

Purity/Purification

Beta Galactosidase antibody was purified by delipidation and defibrination.

Preparation and Storage

Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.

Molecular Weight

74,992 Da

3D Structure

ModBase 3D Structure for Q9TRY9

Biohazard Information

This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Immunogen

Beta galactosidase antibody was raised in rabbit using beta galactosidase (E Coli) as the immunogen.

Product Categories/Family for anti-GLB1 antibody

Proteases; Inhibitors; & Enzymes

Applications Tested/Suitable for anti-GLB1 antibody

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Application Notes for anti-GLB1 antibody

ELISA: 1:65,000
WB: 1:500-1:2,000

Other Notes

Small volumes of anti-GLB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GLB1. It may not necessarily be applicable to this product.

NCBI GI #

83415088

NCBI GeneID

403873

NCBI Accession #

NP_001032730.1

UniProt Primary Accession #

Q9TRY9

UniProt Secondary Accession #

O62800; Q3HTI1

UniProt Related Accession #

Q9TRY9

GenBank Nucleotide #

NM_001037641.1

NCBI Official Full Name

beta-galactosidase

NCBI Official Symbol

GLB1

UniProt Protein Name

Beta-galactosidase

UniProt Synonym Protein Names

Acid beta-galactosidase; Lactase

Protein Family

Beta-galactosidase

UniProt Gene Name

GLB1

UniProt Synonym Gene Names

Lactase

UniProt Entry Name

BGAL_CANFA

NCBI Protein Information

beta-galactosidase

UniProt Comments for GLB1

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.

Research Articles on GLB1

1. This is the first study that simultaneously examine the effect of GLB1(+/+), GLB1(+/-) and GLB1(-/-) genotypes on the expression of lysosomal multienzyme complex components.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GLB1 antibody

Reference Product	PubMed Publications
NEU1 antibody	>12 publications with GLB1 and NEU1
CTSA antibody	>6 publications with GLB1 and CTSA
GLA antibody	>3 publications with GLB1 and GLA
HEXB antibody	>3 publications with GLB1 and HEXB
GALNS antibody	>3 publications with GLB1 and GALNS
GUSB antibody	>2 publications with GLB1 and GUSB
GBA antibody	>1 publications with GLB1 and GBA

Pathways associated with anti-GLB1 antibody

Products by Pathway	Pathway Diagram
Asparagine N-linked Glycosylation Pathway antibodies	Asparagine N-linked Glycosylation Pathway Diagram
Biosynthesis Of The N-glycan Precursor (dolichol Lipid-linked Oligosaccharide, LLO) And Transfer To A Nascent Protein Pathway antibodies	Biosynthesis Of The N-glycan Precursor (dolichol Lipid-linked Oligosaccharide, LLO) And Transfer To A Nascent Protein Pathway Diagram
Galactose Metabolism Pathway antibodies	Galactose Metabolism Pathway Diagram
Galactose Metabolism Pathway antibodies	Galactose Metabolism Pathway Diagram
Glycosaminoglycan Degradation Pathway antibodies	Glycosaminoglycan Degradation Pathway Diagram
Glycosaminoglycan Degradation Pathway antibodies	Glycosaminoglycan Degradation Pathway Diagram
Glycosaminoglycan Metabolism Pathway antibodies	Glycosaminoglycan Metabolism Pathway Diagram
Glycosphingolipid Biosynthesis - Ganglio Series Pathway antibodies	Glycosphingolipid Biosynthesis - Ganglio Series Pathway Diagram
Glycosphingolipid Biosynthesis - Ganglio Series Pathway antibodies	Glycosphingolipid Biosynthesis - Ganglio Series Pathway Diagram
Glycosphingolipid Metabolism Pathway antibodies	Glycosphingolipid Metabolism Pathway Diagram

Diseases associated with anti-GLB1 antibody

Disease Name	Pubmed Publications
Gangliosidosis, GM1 Antibodies	>52 publications with GLB1 and Gangliosidosis, GM1
Nervous System Diseases Antibodies	>44 publications with GLB1 and Nervous System Diseases
Mucopolysaccharidosis IV Antibodies	>12 publications with GLB1 and Mucopolysaccharidosis IV
Disease Models, Animal Antibodies	>5 publications with GLB1 and Disease Models, Animal
Adenocarcinoma Antibodies	>3 publications with GLB1 and Adenocarcinoma
Kidney Diseases Antibodies	>2 publications with GLB1 and Kidney Diseases
Weight Loss Antibodies	>2 publications with GLB1 and Weight Loss
Inflammation Antibodies	>2 publications with GLB1 and Inflammation
Atrophy Antibodies	>2 publications with GLB1 and Atrophy
Cardiovascular Diseases Antibodies	>2 publications with GLB1 and Cardiovascular Diseases

Organs/Tissues associated with anti-GLB1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>10 publications with GLB1 and Brain
Heart Antibodies	>2 publications with GLB1 and Heart
Mixed Antibodies	>1 publications with GLB1 and Mixed
Artery Antibodies	>1 publications with GLB1 and Artery