$Western Blot (Western Blot analysis using Carboxypeptidase Y antibodyBoth the antiserum and IgG fractions of anti-Carboxypeptidase Y are shown to detect under reducing conditions of SDS-PAGE the 61 KDa enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5, 000 dilution of the primary antibody is used followed by detection using Goat anti Rabbit IgG (H + L) (HRP) diluted 1:4, 000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results. )$

Western Blot analysis using Carboxypeptidase Y antibody
Both the antiserum and IgG fractions of anti-Carboxypeptidase Y are shown to detect under reducing conditions of SDS-PAGE the 61 KDa enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5, 000 dilution of the primary antibody is used followed by detection using Goat anti Rabbit IgG (H + L) (HRP) diluted 1:4, 000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.

Carboxypeptidase Y (CPY) Antibody – Rabbit Polyclonal

Name: CPY Antibody; Polyclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS535268
Price: 1830 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS535268

MBS535268 is an antibody targeting CPY. Also known as Carboxypeptidase Y antibody. Reactive in Saccharomyces cerevisiae carboxypeptidase Y. Host: Rabbit. Rabbit polyclonal Carboxypeptidase Y antibody More details

$1830 / 0.1 mg +2 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Carboxypeptidase Y (CPY), Polyclonal Antibody

Full Product Name

Carboxypeptidase Y antibody

Product Gene Name

anti-CPY antibody

Product Synonym Names

Polyclonal Carboxypeptidase Y; Anti-Carboxypeptidase Y; Carboxypeptidase YSCY; Carboxypeptidase Y; CPY1; Vacuolar carboxypeptidase Y; LBC1; PRC1; YMR297W

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Saccharomyces cerevisiae carboxypeptidase Y

Form/Format

0.02M K3PO4, pH 7.2, with 0.15M NaCl and 0.01% NaN3.

Purity/Purification

Carboxypeptidase Y antibody was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Specificity

Saccharomyces cerevisiae carboxypeptidase Y

Preparation and Storage

Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.

Molecular Weight

60,270 Da

Biohazard Information

This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Immunogen

Carboxypeptidase Y antibody was raised in rabbit using Carboxypeptidase Y [Baker's Yeast] as the immunogen.

Product Categories/Family for anti-CPY antibody

Proteases; Inhibitors; & Enzymes

Applications Tested/Suitable for anti-CPY antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-CPY antibody

ELISA: 10,000-1:42,000
WB: 1:5,000

Rabbit Anti-CPY Polyclonal Antibody – Cat# MBS535268

Other Notes

Small volumes of anti-CPY antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CPY. It may not necessarily be applicable to this product.

NCBI GI #

18152939

NCBI Accession #

AAB68520.2

NCBI Official Full Name

carboxypeptidase Y

UniProt Protein Name

Carboxypeptidase Y

UniProt Synonym Protein Names

Carboxypeptidase Y

Protein Family

Carboxypeptidase Y-deficient protein

UniProt Gene Name

CPY

UniProt Entry Name

O14414_PICAN

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.