Rabbit Anti-p27 Polyclonal Antibody – Cat# MBS535738

Western Blot analysis using p27 antibody
Western blot using affinity purified p27 antibody shows detection of p27 protein in MCF7 whole cell lysate (lanes 1-3) (arrowhead). Separation was achieved using a 4-20% gradient gel. Blocking occurred using 5% BLOTTO. Primary antibody was diluted 1:500 in 1% BLOTTO. The membrane was washed and reacted with a 1:10, 000 dilution of Dylight (TM) 800 conj ugated Goat anti-Rabbit IgG. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M). Other detection systems will yield similar results.

p27 Antibody – Rabbit Polyclonal

Name: p27 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS535738
Price: 2000 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS535738

MBS535738 is an antibody targeting p27. Also known as p27 antibody. Reactive in Human p27. Host: Rabbit. Rabbit polyclonal p27 antibody More details

$2000 / 0.1 mL +2 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

p27, Polyclonal Antibody

Full Product Name

p27 antibody

Product Gene Name

anti-p27 antibody

Product Synonym Names

Polyclonal p27; Anti-p27; Cyclin-dependent kinase inhibitor p27; p -27; p27; Cyclin-dependent kinase inhibitor 1B; p27Kip1; p 27

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human p27

Form/Format

0.02M K3PO4, pH 7.2, with 0.15M NaCl, and 0.01% NaN3.

Purity/Purification

p27 antibody was purified by delipidation and defibrination.

Concentration

80 mg/ml (lot specific)

Specificity

Human p27

Preparation and Storage

Store at -20 degree C. Aliquot and freeze at -20 degree C or below for long term storage. This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freeze/thaw cycles.

Molecular Weight

21,474 Da

OMIM

606044

Biohazard Information

This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Immunogen

p27 antibody was raised in rabbit using a full length recombinant human p27 protein as the immunogen.

Product Categories/Family for anti-p27 antibody

Cell Cycle & Cell Death

Applications Tested/Suitable for anti-p27 antibody

ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)

Application Notes for anti-p27 antibody

ELISA: 1:10,000-1:40,000
IP: 1:100
WB: 1:1,000-1:4,000

Other Notes

Small volumes of anti-p27 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for p27. It may not necessarily be applicable to this product.

NCBI GI #

2982673

NCBI Accession #

BAA25263.1

NCBI Official Full Name

p27

UniProt Protein Name

Sjoegren syndrome/scleroderma autoantigen 1

UniProt Synonym Protein Names

Autoantigen p27

Protein Family

IAP-like protein

UniProt Gene Name

SSSCA1

UniProt Entry Name

SSA27_HUMAN

UniProt Comments for p27

SSSCA1: Might play a role in mitosis. Antigenic molecule. Could be a centromere-associated protein. May induce anti-centromere antibodies.

Chromosomal Location of Human Ortholog: 11q13.1

Molecular Function: identical protein binding; protein binding

Biological Process: mitosis; cell division

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.