Natriuretic Receptor C / PGCC (PGCC) Antibody – Rabbit Polyclonal

Particulate Guanylate Cyclase C Antibody

The intracellular levels of Cyclic GMP (cGMP) are maintained by the activity of opposing enzymes: synthesizing gualylyl cyciases (GC) and hydrolyzing phosphodiesterases (PDEs). The synthesizing enzymes (GCs) are found in two forms: cytosolic (soluble) and membrane-bound (particulate). While they share similar structural characteristics, they differ in their mechanisms of physiological regulations. Most importantly, soluble GC (sGC) contains a heme group and binds nitric oxide that activates the enzyme, while particulate GC (PGCs) are stimulated by natriuretic peptides. Particulate forms of guanylyl cyclases have been shown to function as natriuretic peptide receptors. In response to G-protein coupled receptor stimulation, the cGMP can be produced from GTP by either soluble guanylate cyclase (sGC), or by PGC. The sGC are heterodimers (a & b polypeptide chains), that are stimulated by nitric oxide and carbon monoxide or by particulate membrane-bound guanylyl cyclases which are activated by a complex mechanism by natriuretic peptides. There is significant structural homology among various PGCs: there is a large N-terminal extracellular domain(ECD), a single transmembrane domain, a large intracellular domain with protein kinase activity (KLD), a C-terminal catalytic domain (CD) and in between is a dimmerization domain (DD). Guanylyl cyclase C (PGC-C), a member of membrane-bound guanylyl cyclases, is a receptor protein for guanylin and uroguanylin. The binding of a ligand to the extracellular domain of PGC-C triggers signal transduction, resulting in the regulation of intestinal fluids and electrolytes (1). A previous study proposed that a ligand-binding site on PGC-C is localized near the transmembrane region. The PGC-C type guanylate cyclase is a receptor for heat stable enterotoxin. PGC-C protein contains an extra-cellular amino acid sequence that is divergent from other PGCs, but retains several structural motifs (KLD, ECD and TMD). Disrupted activity of PGC-C may be involved in acute diarrhea.

The Anti-PGC-C-selective antibodies were generated against conserved sequences near the C-terminal end of the protein that are unique to PGC-C protein. The PGC-C-selective antibodies are affinity purified against immobilized antigen based affinity chromatography that yielded epitope-specific antibodies. Antigenic blocking peptides (P-PGCC) and westem blot positive control (PC-PGCC) in rearly to use SDS-sample buffer are available. The PGC-C antibodies label a 127-130 kDa protein in various tissues including intestine kidney and spleen. Antibodies can be conjugated to fluorophores and other secondary enzymes upon request at nominal cost.

Small volumes of anti-PGCC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS540091 is an antibody targeting PGCC. Also known as Rabbit Polyclonal PGCC antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.