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Phospho-PDE4A (PDE4A) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS540102

Affinity Purified Phosphorylated Phosphodiesterase 4A (S140) AntibodyHydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. More details

$610 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Phospho-PDE4A, Polyclonal Antibody
Full Product Name
Phospho-PDE4A Antibody
Product Gene Name
Product Synonym Names
cAMP specific 3', 5' cyclic phosphodiesterase 4D; DKFZp686M11213; 5''-cyclic phosphodiesterase 4D; cAMP specific 3' 5' cyclic phosphodiesterase 4D; cAMP specific phosphodiesterase 4D; cAMP specific phosphodiesterase PDE4D6; cAMP-specific 3''; cyclic AMP phosphodiesterase; DPDE 3; FLJ97311; HSPDE4D; PDE 43; Pde4d; PDE4DN2; Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3); Phosphodiesterase 4D cAMP specific; Phosphodiesterase E3 dunce homolog Drosophila; STRK 1; STRK1 antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rabbit, Rat
Form/Format
Affinity Purified
Concentration
0.57 ug/ul in antibody stabilization buffer (lot specific)
Specificity
This antibody detects all known long-form variants of PDE4A, including PDE4A4, PDE4A5, PDE4A8, PDE4Ax when phosphorylated at the PKA phosphorylation site. It will not cross-react with PDE4A phosphorylated at the ERK phosphorylation site. It does not cross-react with other members of the PDE family including PDE4B or PDE4D.
Preparation and Storage
-20 degree C for long term storage
Matching Pairs
Antibody/Peptide Pairs
Immunogen
Synthetic peptide corresponding to PKA phosphorylation site on PDE4A long form enzymes. The peptide was synthesized with phosphorylated serine residue.
Expression
Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
Modification
Phosphorylated
Molecular Function
Hydrolase, 3', 5'-cyclic-AMP phosphodiesterase activity, cAMP binding
Structure
Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1).
Subcellular Location
Apical cell membrane, Cytoplasm, Membrane
Applications Tested/Suitable for anti-PDE4A antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-PDE4A antibody
Dot Blot: 1:10,000
ELISA: 1:10,000
Immunoprecipitation: 1:150
Western Blot: 1:500

Western Blot (WB) of anti-PDE4A antibody

Rabbit Anti-PDE4A Polyclonal Antibody – Cat# MBS540102

Western Blot (WB) of anti-PDE4A antibody

Rabbit Anti-PDE4A Polyclonal Antibody, Western Blot (WB) – Cat# MBS540102

Western Blot (WB) of anti-PDE4A antibody

Rabbit Anti-PDE4A Polyclonal Antibody, Western Blot (WB) – Cat# MBS540102

Other Notes

Small volumes of anti-PDE4A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDE4A purchase

MBS540102 is an antibody targeting PDE4A. Also known as Phospho-PDE4A Antibody. Reactive in Human, Mouse, Rabbit, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PDE4A. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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