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TdT Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS540121

Affinity Purified DNA nucleotidylexotransferase Antibody C-epitopeTemplate-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the… More details

$480 / 0.1 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
TdT, Polyclonal Antibody
Full Product Name
TdT Antibody
Product Gene Name
anti-TdT antibody
Product Synonym Names
Deoxynucleotidyltransferase terminal; DNA nucleotidylexotransferase; DNTT; Nucleosidetriphosphate DNA deoxynucleotidylexotransferase; TDT; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal transferase antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Chicken, Human, Monkey, Mouse, Rat
Form/Format
Affinity Purified
Concentration
0.6 ug/ul in antibody stabilization buffer (lot specific)
Preparation and Storage
-20 degree C for long term storage
Matching Pairs
Antibody: TdT (MBS540121)
Positive Control: TdT (MBS543306)
Molecular Weight
58,408 Da
OMIM
187410
3D Structure
ModBase 3D Structure for P04053
Immunogen
Synthetic peptide taken within amino acid region 450-500 on human Terminal deoxynucleotidyltransferase protein.
Immunoglobulin
IgG
Storage Buffer
Preservative: 0.02% Sodium Azide
Determinant
C-epitope
Molecular Function
Nucleotidyltransferase, Transferase, DNA binding
Structure
Interacts with PRP19 and DNTTIP1. Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNA.
Subcellular Location
Nucleus

Product Categories/Family for anti-TdT antibody

Primary Antibodies; Immunology; TdT Antibody
Applications Tested/Suitable for anti-TdT antibody
Confocal Microscopy (CM), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-TdT antibody
Confocal Microscopy||1:250
Dot Blot: 1:10,000
ELISA: 1:10,000
Immunocytochemistry: 1:250
Immunofluorescence: 1:250
Immunohistochemistry: 1:250
Immunoprecipitation: 1:250
Western Blot: 1:1,000. Detects a band of approximately 58 kDa.

Immunohistochemistry (IHC) of anti-TdT antibody

Rabbit Anti-TdT Polyclonal Antibody – Cat# MBS540121

Immunohistochemistry (IHC) of anti-TdT antibody

Rabbit Anti-TdT Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS540121

Other Notes

Small volumes of anti-TdT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TdT purchase

MBS540121 is an antibody targeting TdT. Also known as TdT Antibody. Reactive in Chicken, Human, Monkey, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TdT. It may not necessarily be applicable to this product.

NCBI GI #
63054850
NCBI GeneID
1791
NCBI Accession #
NP_004079
UniProt Primary Accession #
P04053
UniProt Secondary Accession #
Q53FH1; Q5W103; Q96E50
UniProt Related Accession #
P04053
GenBank Nucleotide #
NM_004088.3
NCBI Official Full Name
DNA nucleotidylexotransferase isoform 1
NCBI Official Synonym Full Names
DNA nucleotidylexotransferase
NCBI Official Symbol
DNTT
NCBI Official Synonym Symbols
TDT
UniProt Protein Name
DNA nucleotidylexotransferase
UniProt Synonym Protein Names
Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal transferase
Protein Family
Tonoplast dicarboxylate transporter
UniProt Gene Name
DNTT
UniProt Synonym Gene Names
; Terminal transferase
UniProt Entry Name
TDT_HUMAN
NCBI Protein Information
DNA nucleotidylexotransferase

NCBI Summary for TdT

This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]
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UniProt Comments for TdT

DNTT: Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. Belongs to the DNA polymerase type-X family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; EC 2.7.7.31

Chromosomal Location of Human Ortholog: 10q23-q24

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: DNA binding; DNA nucleotidylexotransferase activity; DNA-directed DNA polymerase activity; metal ion binding; protein binding

Biological Process: DNA metabolic process; DNA modification
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Research Articles on TdT

1. Absence of TdT expression identifies a subset of high-risk T-acute lymphoblastic leukemia/lymphoma that overlaps with, but is not identical to, the ETP leukemia, providing additional prognostic value.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-TdT antibody

 Products by Pathway  Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Non-homologous End-joining Pathway antibodies  Non-homologous End-joining Pathway Diagram
 Non-homologous End-joining Pathway antibodies  Non-homologous End-joining Pathway Diagram
 Validated Targets Of C-MYC Transcriptional Repression Pathway antibodies  Validated Targets Of C-MYC Transcriptional Repression Pathway Diagram

Diseases associated with anti-TdT antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >1 publications with TdT and Necrosis

Organs/Tissues associated with anti-TdT antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >8 publications with TdT and Blood
 Thymus Antibodies  >3 publications with TdT and Thymus
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