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Histone H3 (HIST1H3I) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS540878

Affinity Purified Histone H3 AntibodyCore component of nucleosome.Play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. More details

$480 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Histone H3 (HIST1H3I), Polyclonal Antibody
Full Product Name
Histone H3 Antibody
Product Gene Name
Product Synonym Names
H3.3B; H3F3A; H3 histone; H3.3A; H3F3; H3f3b; HIST3H3; Histone H3 3 pseudogene; PP781 antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Monkey
Pedicted: Rat
Form/Format
Affinity Purified
Concentration
0.50ug/ul in antibody stabilization buffer (lot specific)
Preparation and Storage
-20 degree C for long term storage
Matching Pairs
Antibody/Peptide Pairs
Molecular Weight
15,429 Da
3D Structure
ModBase 3D Structure for P69150
Immunogen
Synthetic peptide taken within amino acid region 1-50 on human HH3 protein.
Expression
During Sphase, then expression decreases as cell division slows down during the process of differentiation
Determinant
N-epitope
Molecular Function
DNA-binding
Structure
Belongs to histone H3 family
Subcellular Location
Nucleus; Chromosome
Applications Tested/Suitable for anti-HIST1H3I antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-HIST1H3I antibody
Dot Blot: 1:10,000
ELISA: 1:10,000
Immunohistochemistry: 1:100
Immunoprecipitation: 1:200
Western Blot: 1:500

Western Blot (WB) of anti-HIST1H3I antibody

Rabbit Anti-HIST1H3I Polyclonal Antibody – Cat# MBS540878

Immunohistochemistry (IHC) of anti-HIST1H3I antibody

Rabbit Anti-HIST1H3I Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS540878

Other Notes

Small volumes of anti-HIST1H3I antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HIST1H3I purchase

MBS540878 is an antibody targeting HIST1H3I. Also known as Histone H3 Antibody. Reactive in Human, Monkey
Pedicted: Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HIST1H3I. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
histone H3
NCBI Official Synonym Full Names
histone cluster 1, H3i
NCBI Official Symbol
HIST1H3I
NCBI Official Synonym Symbols
H3.f; H3/f; H3FF
UniProt Protein Name
Histone H3
UniProt Synonym Protein Names
H3S; Histone H3-I/H3-II; Major histone H3
UniProt Gene Name
HHT1
UniProt Entry Name
H31_TETTH
NCBI Protein Information
histone H3.1

NCBI Summary for HIST1H3I

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
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UniProt Comments for HIST1H3I

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H3 is deposited into chromatin exclusively through a DNA replication-coupled pathway that can be associated with either DNA duplication or DNA repair synthesis during meiotic homologous recombination.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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