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FLI1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS541620

FITC-Conjugated Friend leukemia integration 1 transcription factor Antibody N-epitopeSequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'. More details

$610 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
FLI1, Polyclonal Antibody
Full Product Name
FLI1 Antibody FITC
Product Gene Name
Product Synonym Names
ERGB transcription factor; Ewing Sarcoma breakpoint region 2; EWSR 2; FLI 1 proto oncogene; FLI1; FLI1 EWS fusion gene; FLI1 proto oncogene; Friend leukemia integration 1 transcription factor; Friend leukemia virus integration 1; Proto-oncogene Fli-1; SIC1; Transcription factor ERGB; Viral integration region FLI1 antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Cat, Human, Mouse, Pig, Rat
Concentration
0.55-0.75 ug/ul in antibody stabilization buffer (lot specific)
Preparation and Storage
-20 degree C for long term storage
Antibody/Peptide Pairs
FLI1 peptide (MBS543695) is used for blocking the activity of FLI1 antibody (MBS541620)
Molecular Weight
29,084 Da
OMIM
193067
3D Structure
ModBase 3D Structure for Q01543
Immunogen
Synthetic peptide taken within amino acid region 1-50 on human Friend Leukemia integration 1 transcription factor protein.
Immunoglobulin
IgG
Expression
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain.
Determinant
N-epitope
Molecular Function
Chromatin Binding; DNA binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity invovled in positive regulation of transcription.
Structure
Can form homodimers or heterodimers with ETV6/TEL1.
Subcellular Location
Nucleus
Applications Tested/Suitable for anti-FLI1 antibody
Confocal Microscopy (CM), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-FLI1 antibody
ELISA: 1:10,000
Immunocytochemistry: 1:100
Immunofluorescence: 1:100
Immunohistochemistry: 1:100
Immunoprecipitation: 1:150
Western Blot: 1:500

Other Notes

Small volumes of anti-FLI1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FLI1 purchase

MBS541620 is an antibody targeting FLI1. Also known as FLI1 Antibody FITC. Reactive in Cat, Human, Mouse, Pig, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FLI1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
FLI-1, partial
NCBI Official Synonym Full Names
Fli-1 proto-oncogene, ETS transcription factor
NCBI Official Symbol
FLI1
NCBI Official Synonym Symbols
EWSR2; SIC-1
UniProt Protein Name
Friend leukemia integration 1 transcription factor
UniProt Synonym Protein Names
Proto-oncogene Fli-1; Transcription factor ERGB
UniProt Gene Name
FLI1
UniProt Entry Name
FLI1_HUMAN
NCBI Protein Information
Friend leukemia integration 1 transcription factor

NCBI Summary for FLI1

This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
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UniProt Comments for FLI1

Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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