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Phospho-P27 KIP1 (p27 KIP1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS543906

FITC-Conjugated Phosphorylated Cyclin-dependent kinase inhibitor p27kip1 Antibody Extreme C-epitopeImportant regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin… More details

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Product Overview

Online Datasheet in PDF
Product Name
Phospho-p27 KIP1, Polyclonal Antibody
Full Product Name
Phospho p27 KIP1 Antibody FITC-Conjugated
Product Gene Name
Product Synonym Names
AA408329; AI843786; CDKN 1B; CDKN 4; Cyclin Dependent Kinase Inhibitor 1B; Cyclin dependent kinase inhibitor p27; KIP 1; MEN1B; MEN4; p27; p27 Kip1; P27-like cyclin-dependent kinase inhibitor antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Chicken, Human, Monkey, Mouse, Rat
Form/Format
FITC-Conjugated
Concentration
0.50-0.75 ug/ul in antibody stabilization buffer (lot specific)
Preparation and Storage
-20 degree C for long term storage
Antibody/Peptide Pairs
Molecular Weight
22,099 Da
3D Structure
ModBase 3D Structure for Q9NYG6
Immunogen
Synthetic peptide taken within amino acid region 148-198 on human Cyclin-dependent kinase inhibitor 1 (p27, KIP1) protein.
Expression
Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.
Modification
Phosphorylated
Determinant
Extreme C-epitope
Molecular Function
Cyclin-dependent protein serine/threonine kinase inhibitor activity, kinase activity
Structure
Forms a ternary complex with CCNE1/CDK2/CDKN1B. Interacts directly with CCNE1; the interaction is inhibited by CDK2-dependent phosphorylation on Thr-187. Interacts with COPS5, subunit of the COP9 signalosome complex; the interaction leads to CDKN1B degrad
Subcellular Location
Nucleus
Applications Tested/Suitable for anti-p27 KIP1 antibody
ELISA (EIA), IMM, Western Blot (WB)
Application Notes for anti-p27 KIP1 antibody
Dot Blot: 1:10,000
ELISA: 1:10,000
Immunoprecipitation: 1:150
Western Blot: 1:250

Other Notes

Small volumes of anti-p27 KIP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for p27 KIP1 purchase

MBS543906 is an antibody targeting p27 KIP1. Also known as Phospho p27 KIP1 Antibody FITC-Conjugated. Reactive in Chicken, Human, Monkey, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for p27 KIP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
Cyclin-dependent kinase inhibitor 1B (p27, Kip1)
NCBI Official Synonym Full Names
cyclin-dependent kinase inhibitor 1B
NCBI Official Symbol
CDKN1B
NCBI Official Synonym Symbols
KIP1; MEN4; CDKN4; MEN1B; P27KIP1
UniProt Protein Name
Cyclin-dependent kinase inhibitor p27kip1
UniProt Synonym Protein Names
Cyclin-dependent kinase inhibitor p27kip1
UniProt Entry Name
Q9NYG6_HUMAN
NCBI Protein Information
cyclin-dependent kinase inhibitor 1B

NCBI Summary for p27 KIP1

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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