Phospho-PDE5A (PDE5A) Antibody – Rabbit Polyclonal

FITC-Conjugated Phosphorylated Phosphodiesterase 5A Antibody
The cyclic monophosphate nucleotides, cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) are found ubiquitously in mammalian cells and act as second messenger transducers to effect the intracellular actions of a variety of G-protein coupled receptors (GPCRs) for hormones, cytokines, and neurotransmitters. Cyclic nucleotides are important intracellular second messengers which play important role in a variety of signal transduction process. The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. One of the many phosphodiesterases that compartmentalized and hydrolyze cGMP in various tissues is phosphodiesterase type 5A (POE5A). The cGMP is involved in nitric oxide signaling as well as cell signaling associated with natriuretic peptides and gulanylins. Some of the intracellular biding cites for the cGMP include cyclic nucleotide gated ion channels, cGMP-dependent protein kinases, and cyclic GMP biding phosphodiesterases (cGB-POEs). The cGB-POEs include POE2, POE3, POE5 and POE10; the members of these families contain various structural and functional motifs that are conserved. Most of these proteins contain dimmeric subunits that contain a highly conserved cGMP binding site and a phosphodiesterase catalytic site. The cGMP-specific phosphodiesterase type-5A (POE5A) family is comprised of single gene with multiple splice variants (POE5A1 and POE5A2) generated by RNA splicing and use the of alternate initiation sites (1,2). POE5 is highly expressed in aorta, lungs, intestine, kidney adrenal gland, cerebellum, and cerebrum. In cerebellum, the POE5 is highly expressed during neonatal development in Purkinje cells layer. POE5 is also abundant in vascular smooth muscle regulating cGMP levels and vascular smooth muscle tonicity. In corpus cavernosum inhibition of POE5 by sildenafil corrects erectile dysfunctions. The nitric oxide donor sodium notropurside (SNP) stimulate POE5 activity by cGMP-dependent kinase phosphorylation (Rybalkin et. aI. , 2002; Murthy 2001). POE5Al has 875 amino acids (99.5 kDa). The amino terminal 142 amino acid of the POE5 gene showed no sequence homology with other POEs and also contained serine 92 that is phosphorylated by cGMP-kinases (MCAllister et aI. , 93). A phospho-specific POE5A antibody was generated against the conserved PKG phosphorylation site.

The affinity purified antibody labels POE5A proteins when they are phosphorylated at Ser92 by PKG. The POE5-selective antibody was generated using cyclic peptide methodology that results in higher titer and specificity (3). The POE5-selective antibodies are generated against peptides from unique sequences on the POE5A gene. The affinity purified antibody labels 99 and 89 kDa POE5A 1 and POE5A2 proteins using POE5A WB positive controls. The POE5A-specific antiserum has no cross reactivity against POE5B protein or any other member of the fast growing family of POEs.

Small volumes of anti-PDE5A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS544220 is an antibody targeting PDE5A. Also known as Phospho PDE5A Antibody FITC-Conjugated. Reactive in Cross-Reactivity: Bovine, Human, Mouse, Pig, Rat, Sheep. Host: Rabbit.