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DNA Polymerase Eta (RAD30) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS544681

BIOTIN-Conjugated Chain B, Yeast DNA polymerase Eta AntibodyDNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an… More details

$610 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DNA polymerase eta (RAD30), Polyclonal Antibody
Full Product Name
DNA polymerase eta Antibody BIOTIN-Conjugated
Product Gene Name
Product Synonym Names
DNA polymerase eta; FLJ16395; FLJ21978; POLH; polymerase DNA directed eta; RAD30; RAD30 homolog A; Xeroderma pigmentosum variant type protein; XPV antibody
Clonality
Polyclonal
Host
Rabbit
Form/Format
BIOTIN-Conjugated
Concentration
0.70-0.98 ug/ul in antibody stabilization buffer (lot specific)
Preparation and Storage
-20 degree C for long term storage
Matching Pairs
Antibody/Peptide Pairs
Molecular Weight
71,515 Da
3D Structure
ModBase 3D Structure for Q04049
Immunogen
Synthetic peptide taken within amino acid region 200-300 on Chain A, Yeast DNA polymerase Eta.
Expression
Induced by UV radiation and heat shock.  The mRNA is stabilized during stationary phase.
Molecular Function
Damaged DNA binding, Metal ion binding, DNA-directed DNA polymerase activity
Structure
Interacts with POL30. This interaction is essential for the polymerase eta function.
Subcellular Location
Nucleus
Applications Tested/Suitable for anti-RAD30 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-RAD30 antibody
Dot Blot: 1:10,000
ELISA: 1:10,000
Western Blot: 1:500

Other Notes

Small volumes of anti-RAD30 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RAD30 purchase

MBS544681 is an antibody targeting RAD30. Also known as DNA polymerase eta Antibody BIOTIN-Conjugated. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RAD30. It may not necessarily be applicable to this product.

NCBI GI #
UniProt Primary Accession #
UniProt Secondary Accession #
NCBI Official Full Name
Chain A, Yeast Dna Polymerase Eta
UniProt Protein Name
DNA polymerase eta
UniProt Synonym Protein Names
Radiation-sensitive protein 30
Protein Family
UniProt Gene Name
RAD30
UniProt Synonym Gene Names
DBH1
UniProt Entry Name
POLH_YEAST

UniProt Comments for RAD30

DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. Efficiently incorporates nucleotides opposite to other UV or oxidative DNA damages like O(6)-methylguanine, 7,8-dihydro-8-oxoguanine, 2,6-diamino-4-hydroxy-5-formamidopyrimidine of 2'-deoxyguanosine (FaPydG), or p-benzoquinone DNA adducts.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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