ROCK2 Antibody – Rabbit Polyclonal

The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]

ROCK2: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Homodimer. Interacts with IRS1, RHOB and RHOC. Interacts with RHOA (activated by GTP), PPP1R12A, CHORDC1, SORL1, EP300 and BRCA2. Interacts with NPM1 and this interaction enhances its activity. Interacts with RAF1. Activated by RHOA binding. Inhibited by Y-27632. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Protein type: Protein kinase, AGC; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; AGC group; DMPK family; ROCK subfamily

Chromosomal Location of Human Ortholog: 2p24

Cellular Component: centrosome; cytosol; nucleus; plasma membrane

Molecular Function: ATP binding; metal ion binding; protein binding; protein serine/threonine kinase activity; Rho GTPase binding; structural molecule activity

Biological Process: centrosome duplication; cortical actin cytoskeleton organization and biogenesis; cytokinesis; ephrin receptor signaling pathway; I-kappaB kinase/NF-kappaB cascade; negative regulation of angiogenesis; positive regulation of protein amino acid phosphorylation; protein amino acid phosphorylation; regulation of actin cytoskeleton organization and biogenesis; regulation of cell adhesion; regulation of circadian rhythm; regulation of focal adhesion formation; regulation of keratinocyte differentiation; regulation of stress fiber formation; Rho protein signal transduction; rhythmic process; smooth muscle contraction; vascular endothelial growth factor receptor signaling pathway