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Shade (shd) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS544873

BIOTIN-Conjugated Shade, isoform B Antibody C-epitopeRequired for CNS development; midline glial cells. Involved in the metabolism of insect hormones; responsible for all ecdysone 20-monooxygenase activity during embryonic, larval and adult stages. May be… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Shade (shd), Polyclonal Antibody
Full Product Name
Shade Antibody BIOTIN-Conjugated
Product Gene Name
Product Synonym Names
SH2 domain-containing adapter protein D; SHD; Src homology 2 domain containing transforming protein D antibody Ecdysone 20-monooxygenase
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Drosophila melanogaster
Form/Format
BIOTIN-Conjugated
Concentration
0.55-0.68 ug/ul in antibody stabilization buffer (lot specific)
Preparation and Storage
-20 degree C for long term storage
Antibody/Peptide Pairs
Shade peptide (MBS544466) is used for blocking the activity of Shade antibody (MBS544873)
Molecular Weight
49,017 Da
3D Structure
ModBase 3D Structure for Q9VUF8
Immunogen
Synthetic peptide taken within amino acid region 160-230 on Spook protein.
Expression
Strong expression by embryonic stage 10 in epidermis, decreases significantly in older embryos. Third instar larvae show expression in the midgut copper cells, Malpighian tubules and fat body. In the adult ovaries, expression is seen in both nurse cells a
Determinant
C-epitope
Molecular Function
Monooxygenase, Oxidoreductase
Structure
Belongs to the cytochrome P450 family.
Subcellular Location
Mitochondrion membrane
Applications Tested/Suitable for anti-shd antibody
Confocal Microscopy (CM), ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-shd antibody
Confocal Microscopy||1:200
Dot Blot: 1:10,000
ELISA: 1:10,000
Immunocytochemistry: 1:200
Immunofluorescence: 1:200
Immunohistochemistry: 1:200
Western Blot: 1:500

Other Notes

Small volumes of anti-shd antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for shd purchase

MBS544873 is an antibody targeting shd. Also known as Shade Antibody BIOTIN-Conjugated. Reactive in Drosophila melanogaster. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for shd. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
shade, isoform B
NCBI Official Synonym Full Names
shade
NCBI Official Symbol
shd
NCBI Official Synonym Symbols
314a1; CG13478; cyp314a1; Cyp314a1; CYP314A1; DmelCG13478
UniProt Protein Name
Ecdysone 20-monooxygenase
UniProt Synonym Protein Names
CYPCCCXIVA1; Cytochrome P450 314a1, mitochondrial; Protein shade
UniProt Gene Name
shd
UniProt Synonym Gene Names
CYP314A1; E20MO
UniProt Entry Name
CP314_DROME
NCBI Protein Information
CG13478 gene product from transcript CG13478-RD

UniProt Comments for shd

Required for CNS development; midline glial cells. Involved in the metabolism of insect hormones; responsible for all ecdysone 20-monooxygenase activity during embryonic, larval and adult stages. May be involved in the breakdown of synthetic insecticides.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-shd antibody

 Reference Product  PubMed Publications
 sad antibody  >5 publications with shd and sad

Organs/Tissues associated with anti-shd antibody

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