• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Shadow (sad) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS544880

BIOTIN-Conjugated Shadow Antibody N-epitopeRequired for CNS development: midline glial cells. Involved in the metabolism of insect hormones: responsible for ecdysteroid C2-hydroxylase activity. May be involved in the breakdown of synthetic insecticides. More details

$610 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Shadow (sad), Polyclonal Antibody
Full Product Name
Shadow Antibody BIOTIN-Conjugated
Product Gene Name
Product Synonym Names
CG14728; Cyp315a1; CYPCCCXVA1; Cytochrome P450 315a1 mitochondrial; Karmoisin ghost; LP13573p; sad; Sad PA; shade antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit Fly)
Form/Format
BIOTIN-Conjugated
Concentration
0.7 ug/ul in antibody stabilization buffer (lot specific)
Preparation and Storage
-20 degree C for long term storage
Antibody/Peptide Pairs
Shadow peptide (MBS544469) is used for blocking the activity of Shadow antibody (MBS544880)
Molecular Weight
59,631 Da
3D Structure
ModBase 3D Structure for Q9VGH1
Immunogen
Synthetic peptide corresponding to amino acids 302-320 of D. melanogaster Shadow. Peptide was conjugated to KLH.
Sequence: CIRVQEDQRRPHDEALYHR
Expression
Complex coexpression pattern of dib (disembodied) and sad (shade) in the early embryo that restricts to the prothoracic gland cells of the developing ring gland during late embryogenesis. In larvae and adult, coexpression is seen in prothoracic gland and follicle cells of the ovary. In adults, coexpression is seen in the follicle cells, sad only is expressed in nurse cells.
Determinant
N-epitope
Molecular Function
Monooxygenase, Oxidoreductase, ecdysteroid 2-hydroxylase activity
Subcellular Location
Mitochondrion membrane
Applications Tested/Suitable for anti-sad antibody
Confocal Microscopy (CM), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-sad antibody
Confocal Microscopy||1:200
Dot Blot: 1:10,000
ELISA: 1:10,000
Immunocytochemistry: 1:200
Immunohistochemistry: 1:200
Immunoprecipitation: 1:200
Western Blot: 1:500

Other Notes

Small volumes of anti-sad antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for sad purchase

MBS544880 is an antibody targeting sad. Also known as Shadow Antibody BIOTIN-Conjugated. Reactive in Drosophila melanogaster (Fruit Fly). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for sad. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
shadow
NCBI Official Synonym Full Names
shadow
NCBI Official Symbol
sad
NCBI Official Synonym Symbols
315a1; CG14728; Cyp315a1; CYP315A1; DmelCG14728; Sad
UniProt Protein Name
Cytochrome P450 315a1, mitochondrial
UniProt Synonym Protein Names
CYPCCCXVA1; Protein shadow
Protein Family
UniProt Gene Name
sad
UniProt Synonym Gene Names
Cyp315a1
UniProt Entry Name
CP315_DROME
NCBI Protein Information
CG14728 gene product from transcript CG14728-RA

UniProt Comments for sad

Required for CNS development: midline glial cells. Involved in the metabolism of insect hormones: responsible for ecdysteroid C2-hydroxylase activity. May be involved in the breakdown of synthetic insecticides.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-sad antibody

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000