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Fibroblast Growth Factor-Basic Recombinantmurine Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS546070

FGF basic (FGF-2, HBGF-2) is one of at least 22 mitogenic proteins of the FGF family, which show 35 - 60% amino acid conservation. Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. Storage pools within the… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Fibroblast Growth Factor-basic, Active Protein
Full Product Name
RecombinantMurine Fibroblast Growth Factor-basic (rMu bFGF)
Product Synonym Names
Fibroblast Growth Factor-basic; murine FGF-basic; Murine Fibroblast Growth Factor-basic
Host
E Coli
Form/Format
Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder
Purity/Purification
>98% by SDS-PAGE and HPLC analyses
Preparation and Storage
This lyophilized preparation is stable at 2-8 degree C, but should be kept at -20 degree C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 degree C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degree C to -70 degree C. Avoid repeated freeze/thaw cycles.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Molecular Weight Information
Approximately 16 kDa, a single non-glycosylated polypeptide chain containing 136 amino acids.
Biological Activity
Fully biologically active when compared to standard. The ED50, as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is <0.1ng/ml, corresponding to a specific activity of of > 1x107 units/mg
Endotoxin Level
Less than 1EU/mg of rMubFGF as determined by LAL method
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20 degree C. Further dilutions should be made in appropriate buffered solutions

Other Notes

Small volumes of Fibroblast Growth Factor-basic active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Fibroblast Growth Factor-basic purchase

MBS546070 is an active protein targeting Fibroblast Growth Factor-basic. Also known as RecombinantMurine Fibroblast Growth Factor-basic (rMu bFGF). Host: E Coli.
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