Human CSF3 Active Protein – Cat# MBS546078

Granulocyte Colony Stimulating Factor (CSF3) Human Active Protein

Name: CSF3 Active Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS546078
Price: 290 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS546078

G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Granulocyte Colony Stimulating Factor (CSF3), Active Protein

Full Product Name

Recombinant Human Granulocyte Colony Stimulating Factor (rHu G-CSF)

Product Gene Name

CSF3 active protein

Product Synonym Names

Granulocyte Colony Stimulating Factor; human G-CSF; Human Granulocyte Colony Stimulating Factor

Host

E Coli

Form/Format

Lyophilized from a 0.2mm filtered concentrated (1mg/ml) solution in 10mM sodium acetate buffer pH 4.0.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Purity/Purification

>98% by SDS-PAGE and HPLC analyses.

Preparation and Storage

This lyophilized preparation is stable at 2-8 degree C, but should be kept at -20 degree C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 degree C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degree C to -70 degree C. Avoid repeated freeze/thaw cycles.

Molecular Weight

22,293 Da

Chromosome Location

Chromosome: 17; NC_000017.10 (38171614..38174066). Location: 17q11.2-q12

OMIM

138970

3D Structure

ModBase 3D Structure for P09919

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Molecular Weight Information

Approximately 18.6kDa, a single non-glycosylated polypeptide chain containing 161 amino acids.

Biological Activity

Fully biologically active when compared to standard. The ED50 as calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3Hthymidine uptake) is less than 0.1 ng/ml, corresponding to a Specific Activity of 1 x 108 IU/mg.

Endotoxin Level

Less than 1EU/mg of rHuG-CSF as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20 degree C. Further dilutions should be made in appropriate buffered solutions.

Other Notes

Small volumes of CSF3 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CSF3 purchase

MBS546078 is an active protein targeting CSF3. Also known as Recombinant Human Granulocyte Colony Stimulating Factor (rHu G-CSF). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for CSF3. It may not necessarily be applicable to this product.

NCBI GI #

183041

NCBI GeneID

1440

NCBI Accession #

AAA35882.1

UniProt Primary Accession #

P09919

UniProt Secondary Accession #

A8MXR7

UniProt Related Accession #

P09919

NCBI Official Full Name

granulocyte colony stimulating factor

NCBI Official Synonym Full Names

colony stimulating factor 3 (granulocyte)

NCBI Official Symbol

CSF3

NCBI Official Synonym Symbols

GCSF; CSF3OS; C17orf33

UniProt Protein Name

Granulocyte colony-stimulating factor

UniProt Synonym Protein Names

Pluripoietin

Protein Family

Granulocyte colony-stimulating factor

UniProt Gene Name

CSF3

UniProt Synonym Gene Names

C17orf33; GCSF; G-CSF

UniProt Entry Name

CSF3_HUMAN

NCBI Protein Information

granulocyte colony-stimulating factor; filgrastim; lenograstim; pluripoietin

NCBI Summary for CSF3

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

UniProt Comments for CSF3

G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cell cycle regulation; Cytokine

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space

Molecular Function: enzyme binding; growth factor activity; cytokine activity; granulocyte colony-stimulating factor receptor binding

Biological Process: positive regulation of myeloid cell differentiation; positive regulation of protein binding; granulocyte differentiation; multicellular organismal development; cytokine and chemokine mediated signaling pathway; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; immune response; positive regulation of transcription from RNA polymerase II promoter

Research Articles on CSF3

1. HNF1A gene was associated with C-reactive protein, and the region including PSMD3 and CSF3 genes was associated with white blood cell count.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CSF3 active protein

Reference Product	PubMed Publications
CSF2 active protein	>16 publications with CSF3 and CSF2
CSF3R active protein	>11 publications with CSF3 and CSF3R
MMP9 active protein	>6 publications with CSF3 and MMP9
IL10 active protein	>5 publications with CSF3 and IL10
CXCL12 active protein	>3 publications with CSF3 and CXCL12
STAT3 active protein	>2 publications with CSF3 and STAT3
SELL active protein	>1 publications with CSF3 and SELL
CXCR4 active protein	>1 publications with CSF3 and CXCR4

Pathways associated with CSF3 active protein

Products by Pathway	Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokines And Inflammatory Response Pathway antibodies	Cytokines And Inflammatory Response Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Malaria Pathway antibodies	Malaria Pathway Diagram
Malaria Pathway antibodies	Malaria Pathway Diagram
PI3K-Akt Signaling Pathway antibodies	PI3K-Akt Signaling Pathway Diagram

Diseases associated with CSF3 active protein

Disease Name	Pubmed Publications
Disease Susceptibility Antibodies	>6 publications with CSF3 and Disease Susceptibility
Nervous System Diseases Antibodies	>4 publications with CSF3 and Nervous System Diseases
Neutropenia Antibodies	>3 publications with CSF3 and Neutropenia
Leukopenia Antibodies	>2 publications with CSF3 and Leukopenia
Anemia Antibodies	>2 publications with CSF3 and Anemia
Leukemia, Myeloid, Acute Antibodies	>2 publications with CSF3 and Leukemia, Myeloid, Acute
Breast Neoplasms Antibodies	>1 publications with CSF3 and Breast Neoplasms
Peripheral Nervous System Diseases Antibodies	>1 publications with CSF3 and Peripheral Nervous System Diseases
Pulmonary Fibrosis Antibodies	>1 publications with CSF3 and Pulmonary Fibrosis
Brain Injuries Antibodies	>1 publications with CSF3 and Brain Injuries

Organs/Tissues associated with CSF3 active protein

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>5 publications with CSF3 and Skin
Muscle Antibodies	>3 publications with CSF3 and Muscle
Lung Antibodies	>3 publications with CSF3 and Lung
Liver Antibodies	>1 publications with CSF3 and Liver
Brain Antibodies	>1 publications with CSF3 and Brain
Uterus Antibodies	>1 publications with CSF3 and Uterus
Uncharacterized Tissue Antibodies	>1 publications with CSF3 and Uncharacterized Tissue