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Vascular Endothelial Growth Factor 165 Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS546099

VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Vascular Endothelial Growth Factor 165, Active Protein
Full Product Name
Recombinant Human VEGF165 (rHuVEGF165)
Product Synonym Names
Vascular Endothelial Growth Factor 165 ; human VEGF165; Human VEGF 165
Host
E Coli
Form/Format
Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
Physical Appearance: Sterile Filtered White Lyophilized (freeze-dried) powder
Purity/Purification
>95% by SDS-PAGE and HPLC analyses.
Preparation and Storage
This lyophilized preparation is stable at 2-8 degree C, but should be kept at -20 degree C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 degree C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degree C to -70 degree C. Avoid repeated freeze/thaw cycles.
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
Fully biologically active when compared to standard. Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1.0-8.0 ng/ml.
Endotoxin Level
Less than 1EU/mg of rHuVEGF165 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should e apportioned into working aliquots and stored at -20 degree C. Further dilutions should be made in appropriate buffered solutions.

Other Notes

Small volumes of Vascular Endothelial Growth Factor 165 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Vascular Endothelial Growth Factor 165 purchase

MBS546099 is an active protein targeting Vascular Endothelial Growth Factor 165. Also known as Recombinant Human VEGF165 (rHuVEGF165). Host: E Coli.
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